
Forensic Science is the application and implementation of scientific methods and techniques for the purpose of justice. It involves analyzing evidence, identify suspects, understand the circumstances of a crime, and establish connections between crime scenes, individuals, and criminal activities.
Committee for establishment of SFSL
Committee on Teaching of forensic science in University
The scope of forensic science is broad and encompasses various scientific disciplines and applications. Here are the primary areas:
Crime Scene Investigation: Involves the identification, documentation, collection, and preservation of evidence at crime scenes, ensuring its integrity for analysis.
Forensic Biology and DNA Analysis: Analyzes biological samples (e.g., blood, saliva, hair) for DNA profiling, crucial for identifying individuals and establishing connections between victims and suspects.
Forensic Chemistry: Focuses on analyzing substances like drugs, poisons, explosives, and other chemicals found at crime scenes to understand their composition and relevance.
Forensic Toxicology: Studies the effects of drugs, alcohol, poisons, and other chemicals in the body to help determine cause of death, impairment, or poisoning.
Forensic Pathology: Examines bodies to determine the cause and manner of death, often involving autopsies and analysis of injuries, disease, or trauma.
Forensic Anthropology: Analyzes human skeletal remains to establish identity, cause of death, and other information, particularly when the body is decomposed.
Forensic Odontology: Uses dental records and bite mark analysis to help identify victims or connect suspects to a crime.
Forensic Entomology: Studies insects and other organisms found on decomposing remains to estimate time of death and other forensic details.
Forensic Ballistics: Examines firearms, bullets, gunshot residues, and related materials to determine how a shooting occurred and identify weapons.
Forensic Document Examination: Analyzes handwriting, typesetting, ink, paper, and other document features to authenticate or detect forgery.
Digital Forensics: Investigates digital devices and data (e.g., computers, smartphones, networks) to recover, analyze, and interpret electronic evidence.
Forensic Psychology: Assesses the mental state of suspects, witnesses, and victims to determine competency, intent, or psychological motives.
Forensic Accounting: Analyzes financial records to detect fraud, embezzlement, or financial misconduct.
Forensic Engineering: Investigates accidents, structural failures, and product malfunctions to determine the cause and potential negligence.
Wildlife Forensics: Applies forensic science techniques to crimes involving animals, such as poaching and illegal trade of endangered species.
Cyber Forensics: Focuses on investigating cybercrimes, including hacking, cyberstalking, and other internet-based crimes.
Environmental Forensics: Analyzes environmental contamination to identify sources of pollution and hold parties accountable for environmental crimes.
Forensic Linguistics: Examines language patterns, word choice, and grammar in written or spoken communication to assess authorship or interpret meaning in legal contexts.
Forensic Archeology: Combines archeological methods with forensic science to uncover and examine remains and artifacts from historic crime scenes or mass graves.

Key aspects of ethics in forensic science:
Objectivity and Impartiality: Forensic scientists must remain unbiased, providing conclusions based solely on evidence, without personal beliefs or external pressures influencing their findings.
Accuracy and Reliability: Forensic analysis should follow established protocols and methods to ensure results are accurate, reproducible, and scientifically valid.
Confidentiality: Sensitive information from cases must be kept confidential to protect the rights and privacy of individuals involved, with data shared only with authorized personnel.
Integrity of Evidence: Evidence must be collected, handled, and stored correctly to prevent contamination, loss, or tampering, maintaining a strict chain of custody.
Honesty in Reporting: Reports should accurately reflect findings, including any limitations or uncertainties, without exaggerating results or making unsupported conclusions.
Competence: Forensic scientists should work within their area of expertise, continuously updating their skills and knowledge to provide reliable analysis.
Avoiding Conflicts of Interest: Scientists should disclose and avoid any conflicts that might compromise their objectivity, such as personal, financial, or professional interests.
Testimony Integrity: When testifying, forensic experts should communicate findings truthfully, clearly, and without bias, ensuring they do not mislead the court.
Respect for Human Rights: All forensic work should respect individuals’ rights, avoid unethical practices, and support justice without discrimination.
Accountability: Forensic scientists should be accountable for their work, open to peer review, and willing to admit and correct errors to maintain public trust in the field.
Each ethical principle ensures that forensic science upholds justice, reliability, and public confidence in legal proceedings.
Physical Evidence: Any tangible items that may be found at the crime scene, on the victims, or on the culprit are referred to as physical evidence.
Trace Evidence: The term “tracing evidence” refers to physical evidence that is present in minute but quantifiable numbers.
In a criminal investigation, the analysis of the evidence acquired at the scene is utilized to support or refute the case’s facts.
Physical evidence can be either:

There are several common search methods, each method is chosen based on the size, type, and nature of the search area and the specific goals of the investigation or study.
Chain of custody is the process of maintaining and documenting the handling, transfer, and storage of evidence from the moment it is collected until it is presented in court or analysis is completed. It ensures that evidence is accounted for and remains uncontaminated and tamper-free, preserving its integrity for legal and investigative purposes.

Crime Scene is the location where a crime has occurred or where evidence of a crime may be found. Its primary function is to serve as the physical space that holds crucial evidence. Crime scene could be static means a single, immovable location where the crime occurred (e.g., a room, building) or it could be dynamic in nature, involving movement, such as in a car chase or where evidence may have been transferred between locations.
Crime scenes vary based on

Preserving a crime scene is crucial to avoid contamination or loss of evidence. Preservation involves careful, methodical steps to secure the scene and protect all physical, biological, and digital evidence.
Steps in Crime Scene Preservation:
Challenges in Crime Scene Preservation:
Criminal investigations aim to systematically collect, analyze, and preserve evidence to reconstruct events and establish facts that identify suspects and victims, ultimately bringing perpetrators to justice. Investigators employ various forensic techniques and methods based on the crime type, scene characteristics, and the nature of the evidence.
The judicial system in India is organized in a hierarchy to address civil and criminal cases, each level having specific types, powers, and jurisdictions. Courts operate under various legal frameworks, such as the Criminal Procedure Code (Cr.P.C.), 1973, and the Indian Evidence Act, 1872, which also define the role of experts and admissibility of evidence.

Supreme Court of India:
High Courts:
District and Sessions Courts:
Subordinate and Magistrate Courts:





NCRB (National Crime Records Bureau)
1. Objectives and Functions
2. Key Publications and Reports
3. Technological Initiatives and Training
4. Importance in Law Enforcement and Policy
Equality before law | Equal protection of laws |
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Offences | Punishments |
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Punitive | Preventive |
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It is a method used to predict the characteristics, behavior, and psychological traits of offenders and victims in a crime investigation. It helps investigators narrow down suspects, understand motives, and anticipate possible future actions by analyzing behavior patterns and crime scene details.
Lie Detection (Polygraphy)

History
Principle
Working
Techniques
During questioning the subject following approach is applied
Limitations


Quality Control (QC) in forensic institutions ensures accuracy, reliability, and integrity in forensic analyses and outcomes. This process involves implementing standardized protocols, regular audits, and performance checks to maintain high standards across laboratories and investigations.
Quality control in forensic institutions not only assures precise and unbiased results but also strengthens the credibility of forensic contributions within the justice system.
Microscopy is the technique of using microscopes to observe objects and areas of objects that cannot be seen with the naked eye. This field is essential in scientific research, especially in biology, materials science, and medical diagnostics.
Components of a Microscopе
A microscope consists of several key components that work together to magnify and resolve fine details in small specimens. Here are the main components and their functions:
1. Eyepiece (Ocular Lens)
The ocular, or eyepiece, is a cylinder comprehending multiple lenses
Its purpose is to converge the image in focus.
The top of the body tube has an eyepiece inserted into it.
Magnification values for eyepieces comprises 2x, 5x and 10x.
2. Objective Lenses
These are the primary lenses located close to the specimen. They usually come in sets (4x, 10x, 40x, 100x) on a rotating nosepiece.
Each objective lens provides a different level of magnification and resolution.
3. Stage
The flat platform where the slide with the specimen is placed.
Most stages have clips to hold the slide in place and allow easy movement to observe different areas.
4. Stage Clips or Mechanical Stage
These hold the slide in position on the stage. Mechanical stages have knobs to move the slide precisely in small increments.
5. Illuminator or Light Source
Provides light to illuminate the specimen. In most modern microscopes, this is an LED or halogen light source.
Older microscopes may have mirrors to reflect ambient light onto the specimen.
6. Condenser
Located below the stage, it focuses light from the illuminator onto the specimen for clearer visualization.
It often has an adjustable diaphragm to control light intensity and contrast.
7. Diaphragm or Iris
A rotating disk under the stage that controls the amount of light reaching the specimen.
Adjusting the diaphragm can improve contrast and resolution, depending on the transparency of the specimen.
8. Nosepiece or Turret
A rotating part that holds the objective lenses and allows quick switching between different magnifications.
9. Focus Adjustment Knobs
Coarse Focus: Moves the stage or objective lenses quickly and is used for general focusing.
Fine Focus: Allows precise adjustments to bring the specimen into sharp focus, especially under high magnification.
10. Body Tube (Head)
Connects the eyepiece to the objective lenses and ensures proper alignment for viewing.
11. Arm
The part connecting the body tube to the base, providing structural support.
It’s the handle used to carry the microscope.
12. Base
The bottom part of the microscope that provides stability.
Together, these components allow microscopes to magnify specimens, focus light on them, and enable detailed observation and analysis. Each part plays a crucial role in the clarity, contrast, and usability of the microscope
Recombination of Light: When these rays exit the specimen, they pass through the analyzer, recombining to produce interference colors that reveal information about the sample’s internal structure.
The comparison microscope operates by bringing together images from two separate specimens into a split view within one eyepiece. The optical bridge merges the images, so they appear as two halves within a single circular field of view. This setup enables examiners to directly compare details like patterns, striations, and markings.
The comparison microscope has become an indispensable tool in forensic science, enabling detailed comparisons that can support or refute evidence in criminal cases. Its ability to present two images simultaneously within one eyepiece provides a highly effective means of identifying matches or discrepancies at a microscopic level.
The stereomicroscope works by using two separate optical paths angled slightly apart. Each path captures an image from a different perspective, and the two images are merged into one by the brain, giving a three-dimensional view. This 3D perspective provides a clear sense of depth, allowing the user to see the topography and structure of the specimen.
Detection: The emitted fluorescent light passes through the dichroic mirror and emission filter, which blocks any remaining excitation light. The filtered fluorescent light then reaches the eyepiece or camera, forming an image.
Background Fluorescence: Autofluorescence from cellular components or the sample matrix can interfere with the specific fluorescence signal, reducing image contrast
Electrons are accelerated to generate a monochromatic beam. When this beam interacts with the specimen, various scattering and emission phenomena occur, enabling high-resolution imaging.
Backscattered Electrons (BSE): Created by elastic scattering, useful for identifying areas with different atomic numbers (e.g., materials contrast).
Secondary Electrons (SE): Created by inelastic scattering near the surface, ideal for SEM imaging and topography studies.
Inner-Shell Ionization: Ejection of secondary electrons as high kinetic energy from the inner shell
X-rays: Generated during electron transitions between energy levels, used in energy-dispersive X-ray spectroscopy (EDX) to determine elemental composition.
Spectrophotometry: A quantitative analytical method used to measure the absorbance or transmittance of light by a substance as a function of wavelength.
A technique used to measure the absorption or transmission of ultraviolet (UV) (200–400 nm) and visible (400–700 nm) light by a sample.
A technique used to study molecular vibrations and identify functional groups by measuring the absorption of infrared light (IR) by a sample.
| Functional Group | Absorption Range |
|---|---|
| O-H (alcohols) | 3200–3600 (broad) |
| N-H (amines) | 3300–3500 |
| C-H (alkanes) | 2800–3000 |
| C=O (carbonyls) | 1650–1750 |
| C=C (alkenes) | 1600–1680 |
| C≡C, C≡N (triple bonds) | 2100–2300 |
A spectroscopic technique used to study vibrational, rotational, and other low-frequency modes in a system by measuring the inelastic scattering of light (Raman scattering).
An analytical technique used to determine the concentration of elements by measuring the absorption of light by free atoms in the gaseous state.
An analytical technique used to determine the concentration of elements in a sample by measuring the light emitted from excited atoms or ions.
| Method | Excitation Source | Sensitivity | Applications |
|---|---|---|---|
| Flame Emission (FES) | Flame | Moderate | Alkali/alkaline earth metals |
| ICP-AES | Plasma (argon) | High | Multi-element analysis, trace metals |
| Arc/Spark Emission | Electrical discharge | High (for solids) | Metals and alloys |
A non-destructive analytical technique used to determine the elemental composition of a sample by measuring gamma rays emitted after neutron irradiation.
Activity (A):
A=Nσϕ(1−e−λt)
Where:
Decay Law:
A(t)=A0e−λt
Electromagnetic radiation with wavelengths ranging from 0.01 nm to 0.01nm corresponding to high photon energies (0.1–100 keV).
X-Ray Fluorescence (XRF) is a non-destructive analytical technique used to determine the elemental composition of materials. It is widely used in forensics, geology, archaeology, environmental science, and material science.
Interaction of X-rays with Matter:
Emission of Fluorescent X-rays:
Detection:
X-ray Source:
Sample Chamber:
Detector:
Processor and Software:
Wavelength Dispersive XRF (WDXRF):
Energy Dispersive XRF (EDXRF):
Solid Samples:
Powder Samples:
Liquid Samples:
Thin Films or Coatings:
Forensic Science:
Geology:
Archaeology:
Environmental Studies:
Industrial Applications:
A technique used to study the atomic structure of crystalline materials by analyzing the diffraction patterns produced when X-rays interact with the crystal lattice.
Bragg’s Law:
nλ=2dsinθ
Where:
Working Mechanism:
XRD Pattern: A graph of intensity (y-axis) vs. 2θ (diffraction angle, x-axis).
Key Features:
Scherrer Equation:
D=Kλ\βcosθ
Where:
Mass Spectroscopy (MS) is an analytical technique used to determine the mass-to-charge ratio (m/z) of ions. It provides detailed information about the molecular weight, structure, and chemical composition of compounds, making it a powerful tool in forensic science, chemistry, biochemistry, and environmental analysis.
Sample Introduction:
Ion Source:
Mass Analyzer:
Detector:
Data System:
Forensic Science:
Biochemistry:
Pharmaceutical Industry:
Environmental Science:
Material Science:
Key Terms in Mass Spectroscopy
GC-MS (Gas Chromatography-Mass Spectrometry):
LC-MS (Liquid Chromatography-Mass Spectrometry):
MALDI-TOF MS:
Tandem MS (MS/MS):
Chromatography is a powerful analytical technique used to separate mixtures into their individual components. It involves the distribution of components between a stationary phase (solid or liquid) and a mobile phase (liquid or gas). The components of the mixture move at different rates depending on their interaction with the stationary phase, allowing for their separation.
Thin Layer Chromatography (TLC) is a simple and rapid chromatographic technique used to separate and identify compounds in a mixture based on their differential interactions with a stationary phase and a mobile phase.
Principle
Procedure
Applications
Rf Values
Gas-Liquid Chromatography (GLC) is a type of gas chromatography in which the stationary phase is a liquid coated on an inert solid support, and the mobile phase is an inert carrier gas. It is widely used to separate and analyze volatile and semi-volatile compounds.
Components of a GLC System
Procedure
Applications
Detectors in GLC
| Detector | Key Features | Applications |
|---|---|---|
| Flame Ionization Detector (FID) | High sensitivity for organic compounds. | Hydrocarbons, alcohols, esters. |
| Thermal Conductivity Detector (TCD) | Universal detector, detects all compounds. | Permanent gases, volatiles. |
| Electron Capture Detector (ECD) | Highly sensitive for electronegative compounds. | Halogenated compounds, pesticides. |
| Mass Spectrometry (GC-MS) | Identifies compounds based on mass. | Complex mixtures, unknown compounds. |
High-Performance Liquid Chromatography (HPLC) is a chromatographic technique used to separate, identify, and quantify components in a mixture using high-pressure liquid as the mobile phase. It offers high resolution and precision for a wide range of compounds.
Based on Mode of Interaction:
Based on Gradient:
| Detector | Key Features | Applications |
|---|---|---|
| UV-Visible Detector | Most common, detects UV-active compounds. | Drugs, organic molecules. |
| Fluorescence Detector | High sensitivity for fluorescent compounds. | Vitamins, polycyclic aromatics. |
| Refractive Index Detector | Universal but low sensitivity. | Carbohydrates, lipids. |
| Mass Spectrometer (LC-MS) | Highly sensitive, identifies based on mass. | Complex biological samples. |
High-Performance Thin Layer Chromatography (HPTLC) is an advanced version of Thin Layer Chromatography (TLC) that offers higher resolution, sensitivity, and automation for the separation and analysis of compounds.
| Aspect | TLC | HPTLC |
|---|---|---|
| Plate Coating | Manual or semi-uniform | Automated and highly uniform |
| Particle Size | Larger | Smaller (improves resolution) |
| Sensitivity | Moderate | High |
| Sample Application | Manual | Automated |
| Quantification | Visual | Quantitative (via densitometry) |
Comparison of Techniques
Feature | TLC | GLC | HPLC | HPTLC |
Stationary Phase | Solid (plate) | Liquid (coated solid support) | Solid (packed column) | Solid (plate) |
Mobile Phase | Liquid (solvent) | Gas | Liquid | Liquid (solvent) |
Sample Type | Non-volatile compounds | Volatile compounds | Non-volatile compounds | Non-volatile compounds |
Resolution | Moderate | High | High | High |
Sensitivity | Moderate | High | Very high | High |
Applications | Qualitative analysis | Volatile compound analysis | Pharmaceutical, clinical, forensic | Qualitative and quantitative analysis |
Cost | Low | Moderate | High | Moderate |
Immunoassays are biochemical methods used to detect and quantify substances such as proteins, hormones, drugs, or pathogens. These methods leverage the specific binding properties of antigens and antibodies.
Principle
The principle of immunoassays is based on the specific binding between an antigen (the target substance) and an antibody. This interaction forms an antigen-antibody complex, which is then detected or quantified using a measurable signal such as color change, fluorescence, or radioactivity.
Key components:
Types of Immunoassays
Techniques
Applications
Advantages
Blood is a vital fluid connective tissue that performs multiple functions, including the transportation of oxygen and nutrients, removal of waste products, immune defense, and regulation of body temperature and pH balance.
Blood is a specialized connective tissue consisting of two major components:
Hematopoiesis
The process of blood cell formation, known as hematopoiesis, occurs in the bone marrow. Stem cells differentiate into various types of blood cells, maintaining a balanced composition.
Plasma is the straw-colored, viscous fluid portion of blood, comprising 55% of blood volume.
Plasma without clotting factors is called serum.
Formed elements constitute the cellular components of blood.
3 Mechanisms
At any given moment the blood present in the circulatory system is distributed as follows:
Presumptive Test | Confirmatory Test |
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Tetramethyl Benzidine (TMB) Test | |
Reagents | Acetate Buffer: Sodium acetate : 5.0g Glacial Acetic Acid : 43.0 ml Deionized Water : 50.0 ml |
Working Solution: TMB : 0.4g Acetate Buffer : 20.0 ml | |
Procedure | 1. Place a cutting or swabbing of the stain on filter paper or spot test paper. 2. A drop of TMB Solution is placed on the stain, followed by a drop of 3% Hydrogen Peroxide. 3. An immediate blue-green colour is a positive test for peroxidase activity, indicative of hemoglobin.
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Phenolphthalein Test (Kastle-Meyer Test) | |
Reagents | Stock Solution: Phenolphthalein : 2.0 g Potassium Hydroxide : 20.0g Distilled Water : 100 ml Zinc Dust : 20.0 g |
Working Solution: [Solution # 1] Ethanol : 10 ml [Solution # 2] Phenolphthalein Stock : 2 ml Distilled Water : 10 ml Ethanol : 2 ml [Solution # 3] 3% Hydrogen Peroxide : 10 m | |
Procedure | 1. A small cutting, swabbing or extract of the suspected bloodstain is placed on filter paper or spot test paper. 2. Two or three drops of Ethanol are placed on the stain. 3. Two drops of working phenolphthalein solution are added to the stain. 4. After waiting to insure that no colour develops at this stage, two or three drops of 3% Hydrogen Peroxide are added. 5. An intense pink colour is a positive test for peroxidase activity, indicative of hemoglobin. This is not a confirmatory test for blood. |
Luminol Test | |
Reagents | Acetate Buffer: Sodium perborate : 0.7g 3-Aminophthal hydrazide : 0.1g Sodium bicarbonate : 5.0g |
Procedure | 1. Take the suspected blood stain 2. Add few amount of Luminol reagent 3. Appearance of fluorescent color indicate positive test of blood. |
Takayama Test | |
Reagents | Standard Glucose Solution (100g/100ml) : 3 ml 10% Sodium hydroxide : 3 ml Pyridine : 3 ml Distilled Water : 7 ml |
Procedure | 1. Place material to be tested on a microscopic slide and cover with a cover slip. 2. Add a drop of Takayama Reagent and allow to flow under the cover slip. 3. Warm slide gently on a hot plate at 650C for 10-20 seconds 4. Allow to cool and observe under microscope at 100X. 5. The appearance of pink needle shaped crystals of pyridine hemochromogen (Pyridine ferroprotoprophyrin) is positive reaction for heme. |
Teichmann’s Test | |
Reagents | Potassium Chloride or : 0.1g Potassium Bromide : 0.1g Potassium Iodine : 0.1g Glacial Acetic Acid : 100 ml |
Procedure | 1. Place material to be tested on a microscopic slide and cover with a cover slip. 2. Let the Reagent flow under the cover slip. 3. Warm slide gently on a hot plate at 650 C for 10-20 seconds. 4. Allow to cool and observe under microscope at 100X. 5. The appearance of brown rhombohedron shaped crystals of ferroprotoprophyrin chloride is a positive reaction for heme. |
Spectroscopic Estimation | |
Reagents | Solution # 1: 0.2% Sodium lauryl sulphate in water Solution # 2: 0.2% Mercaptoethanol in 1% NH3 solution |
Procedure | 1. Put suspected stain(as small as 2mm) in 0.5% Potassium cyanide solution 2. Rest for 15 min and then fiterate. 3. Take in 1cm cell and pass a U-V light in a spectrometer 4. Absorption observed at from 300 – 600 micron. 5. The absorption maximum at 422 milli micron obtained that the presence of cyclohaemoglobin. |
Spectrophotometric Estimation | |
Reagents | Solution # 1: 0.2% Sodium lauryl sulphate in water Solution # 2: 0.2% Mercaptoethanol in 1% NH3 solution |
Procedure | 1. To a 1 cm long stained thread, add 10 ml of Solution # 1. 2. Incubate at 370C for 15-20 minutes. 3. Add 10ml of Solution # 2 and mix. 4. Transfer liquid to a micro capillary cuvette. 5. On a Spectrophotometer, monitor the reaction at 560 mm against a reaction blank until absorption reaches maximum. 6. When the reaction is complete, after 5-10 minutes, scan the sample between 600 and 500 nm. Two peaks, which are clearly defined at 558 nm and 529 nm, indicate the presence of haemoglobin derivatives. |
Precipitin tube method |
1. Six precipitin tubes (number can vary on the number of anti sera used) 2. Put a drop of the bloodstain/tissue extract in the tubes. 3. Carefully add one drop of antiserum for species origin (anti-Human serum, anti- Fowl serum, anti-Dog serum, anti-Cow Serum, anti-Goat serum, etc.) along the walls of tube. 4. Leave undisturbed for 30 minutes at room temperature. 4 5. Carefully examine the white ring at the interface of two solutions. If ppt is formed, it belongs to that specific anti-serum. |
The International Society of Blood Transfusion currently recognizes 47 blood group systems
Blood Group | Antigens on RBCs | Antibodies in Plasma | Donor’s Group |
A | A | Anti B | A, O |
B | B | Anti A | B, O |
AB | A, B | nil | AB, A, B, O |
O | nil | Anti A, B | O |
Blood Group | % in Population |
O+ | 38.6 |
A+ | 27.4 |
B+ | 22.0 |
AB+ | 5.88 |
O- | 2.55 |
A- | 1.99 |
B- | 1.11 |
AB- | 0.36 |
Biosynthesis of Antigens
A1 | A2 |
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Secretors
Preliminary Test | ||
Lattes Crust | Agglutination-based test that utilizes the A, B, and O indicator cells to test the agglutination reaction with its corresponding naturally occurring serum antibodies Not very sensitive and requires a large quantity of blood Not reliable for testing old stains. | |
Confirmatory Test | ||
Absorption–Elution | Highly sensitive and can be used for testing dried bloodstains The antigens are immobilized in a solid phase At low temperatures, the antigens bind to their corresponding antibodies: anti-A antibodies, anti-B antibodies, or anti-O lectins. The excess unbound antibodies are removed by washing, and the bound antibodies are then eluted at higher temperatures The eluted antibodies can then be identified by an agglutination assay using A, B, and O indicator cells. | |
Exchange of nutrients, gases, etc., between the blood and the cells always occur through this fluid.
Acid Phosphate Types
PRELIMINARY TEST | |||
Test | Reagent/Specification | Confirmation | |
Fluorescence | UV to VIS(450 nm to 700 nm) and filters were used | Bluish White | |
Acid Phosphate | Sodium Naphthyl phosphate & Bapthyl Diazo blue B | Purple | |
Florence Test | Choline is detected | Dark brown crystals of choline periodide, generally needle shaped | |
Barberio Test | Spermine is detected | Needle shaped, rhombic & of yellow colour | |
CONFIRMATORY TEST | |||
Acid Phosphatase Isoenzyme Method
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Creatinine Phosphokinase
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Choline And Spermine Test
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Microscopic Examination
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Fluorescence Microscopy
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PRELIMINARY TEST | ||
Test | Reagent/Specification | Confirmation |
Visual Examination | 470 nm | |
Starch–Iodine/Phadebas | Iodine (I2) is used to test for the presence of starch amylose in starch reacts with iodine to form a dark blue complex amylopectin in starch reacts with iodine to form a reddish-purple color In the presence of amylases, starch is broken down to mono- or disaccharides. Consequently, such colors do not develop when iodine is added | Light brown ring Or “not specific to HSA” |
Colorimetric | Dye-labeled amylase substrates (dye-conjugated amylose or amylopectin) substrates are not soluble in water. In the presence of amylase, the dye-containing moieties are cleaved and are soluble in water to produce a color | measured colorimetrically by spectrophotometric methods “not specific to HSA” |
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CONFIRMATORY TESTS | ||
Test | ||
Immunochromatographic | ||
Enzyme-Linked Immunosorbent Assay (ELISA) | ||
RNA-Based Assays | ||
Vagina | |||||
Squamous mucosa |
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Submucosa | |||||
Skin Cells | Buccal Cells | Vaginal Cells |
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PRELIMINARY TEST | ||||||||
Test | Reagent/Specification | Confirmation | ||||||
Lugol’s Iodine Staining | By French physician Jean Lugol. Iodine reacts with glycogen to exhibit color Solution: 5% iodine (I2) + 10% potassium iodide (KI) | |||||||
Periodic Acid–Schiff Staining | Vaginal epithelial cells are stained | Cytoplasm = Magenta Nucleus = Purple | ||||||
Dane’s Staining | Can differentiate skin and vaginal cells | Skin = Red or Orange Buccal = Orange-Pink Nuclei= Red Vaginal = Bright Orange Nuclei= Orange | ||||||
CONFIRMATORY TESTS | ||||||||
Fluorescence spectroscopy | ||||||||
Raman spectroscopy | ||||||||
Reverse transcription polymerase chain reaction (RT-PCR) | ||||||||
RNA-Based Assay | ||||||||
CONFIRMATORY TESTS | ||
D-dimer Assay
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Enzyme-linked immunosorbent assay (ELISA) | ||
Lactate Dehydrogenase Assay
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RNA-Based Assay |
DNA (Deoxyribonucleic Acid) is a biopolymer encoding genetic information in living organisms and some viruses.
Types of DNA
DNA is a Good Genetic Marker
Polymorphism: DNA exhibits variations, such as:
Short Tandem Repeats (STRs): Repeating units of 2-6 base pairs.
Single Nucleotide Polymorphisms (SNPs): Variations at single nucleotide positions.
Universality: Found in almost all cells, including hair, blood, saliva, and bone.
Stability: DNA remains intact under various environmental conditions.
Specialized DNA Types
Visualization Techniques
DNA profiling is the process of identifying individuals based on their unique DNA patterns.
DNA phenotyping, also known as forensic DNA phenotyping (FDP), refers to the process of predicting an individual’s physical appearance or ancestry based on their DNA profile. This involves analyzing specific genetic markers associated with visible traits.
Applications of DNA Phenotyping:
RNA Profiling
Definition:
RNA profiling involves analyzing RNA molecules to understand gene expression patterns, which can help determine the type, state, and timing of biological samples or cellular activity.
Key Features of RNA Profiling:
Applications of RNA Profiling:
DNA Phenotyping vs. RNA Profiling
Feature | DNA Phenotyping | RNA Profiling |
Focus | Prediction of physical traits and ancestry | Analysis of biological activity and tissue type |
Molecular Basis | DNA (genotype) | RNA (gene expression) |
Applications | Physical identification, missing persons, ancestry | Tissue origin, timing of events, activity states |
Stability | Highly stable (over decades or centuries) | Less stable, decays quickly |
Initialization step:
Denaturation step:
Annealing step:
Extension/elongation step:
Final elongation:
Final hold:
Exponential amplification:
Leveling off-stage:
Plateau:
Modifications of basic PCR technique |
Allele-specific PCR :
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Assembly PCR or Polymerase Cycling Assembly (PCA) :
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Asymmetric PCR :
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Helicase-dependent amplification :
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Hot-start PCR:
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Inter sequence-specific PCR (ISSR):
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Inverse PCR:
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Methylation-specific PCR (MSP):
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Mini-primer PCR:
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Multiplex-PCR:
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Nested PCR:
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Quantitative PCR (Q-PCR):
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Reverse Transcription PCR (RT-PCR):
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Thermal asymmetric interlaced PCR (TAIL-PCR):
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Touchdown PCR (Step-down PCR):
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Universal Fast Walking:
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The Wildlife (Protection) Act of 1972 is a landmark legislation in India aimed at protecting the country’s wildlife and biodiversity.
Scope of Wildlife Forensics
Evidence in Wildlife Forensics
Identification in Wildlife Forensics
Significance of Wildlife Forensics
Wildlife forensics is indispensable for combating wildlife crimes, enforcing the Wildlife (Protection) Act, and safeguarding our natural heritage.
Inebriants are substances that cause intoxication by affecting the central nervous system (CNS), leading to altered mental and physical states. These substances induce euphoria, impaired judgment, decreased motor coordination, and other effects typical of intoxication. Inebriants include:
Their effects range from mild stimulation and relaxation to severe CNS depression, depending on the dose and type of substance. Overuse or misuse can result in acute poisoning or chronic health issues.
Beverage | Alcohol by volume |
Spirits (whisky, brandy, rum, gin, vodka) | 35-50% |
Port (fortified with brandy), sherry [Wine + Spirit] | 17-21% |
Wine | 10-15% |
Champagne | 10-13% |
Beers, stout, cider | 4-8% |
Commercial Preparation | Composition |
Absolute alcohol | 99.95% ethanol |
Mineralized methylated spirit | 90% ethanol + 9.5% wood naphtha (methanol) + 0.5% pyridine (colored pink for easy identification) |
Industrial methylated spirit | 95% ethanol + 5% methanol (no coloring agent) |
Surgical spirit | 95% ethanol + 5% methanol (Methyl salicylate aka oil of wintergreen added to give it a sweetish flavor) |
Rectified spirit | 95% ethanol |
Liquors | Composition | Location |
Mahua | Dried flower of mahua tree (Madhuca longifolia) and chhowa gud (granular molasses) | MP, India |
Toddy | Sap of various species of palm tree | Asia and Africa |
Feni | Coconut or juice of cashew apple | Goa, India |
Arrack | Distilled from coco-palm, raisins, rice, sugar or jaggery (may be mixed with chloral hydrate or potassium bromide) | Asia |
Tharra | Fermenting the mash of sugarcane juice/pulp | MP, India |
Chhaang | Rice beer, barley and millet stuffed in a barrel of bamboo | Tibet |
Handia | Fermenting boiled rice mixed with herbs | MP, India |
Chuak | Fermenting rice in water | Tripura |
Sonti | Steaming rice mold, Rhizopus sonti | India |
Blood: Urine | 1:1.35 |
Blood: Exhaled air (breath) | 1:2300 |
Blood: Saliva | 1:12 |
Blood: CSF | 1:1.17 |
Alcohol | Product (Via ADH) | Final Product |
Ethyl Alcohol | Acetaldehyde | Acetic Acid |
Methyl Alcohol | Formaldehyde | Formic Acid |
Isopropyl Alcohol | Acetone | Formate and finally carbon dioxide |
Ethylene Glycol | Glycolaldehyde | Glycolic acid and finally Oxalic acid to Calcium oxalate |
Permissible BAC (mg/dl) | Countries |
0 | Hungary, Pakistan, Saudi Arabia, UAE |
20 | Norway, Poland, Sweden |
30 | India, China, Japan |
50 | Australia, France, Germany, Italy, Spain, Russia |
80 | Canada, UK, US (100 mg/dl in some states) |
a = c p r |
a – total amount of alcohol (in grams) absorbed in body
c – concentration of alcohol in blood (in g/kg)
p – weight of person (in kg)
r – constant (0.68 in men and 0.5 in women)
a = ¾ q p r |
q – concentration of alcohol in urine (in g/l)
Long acting (8-24 h) | Short acting (3-6 h) | Ultra-short acting (0.5-2 h) |
Phenobarbital | Butobarbital | Thiopental |
Mephobarbital | Secobarbital | Methohexital |
Pentobarbital | Hexobarbital | Thiamylal |
Fatal dose and blood level | ||
Category | Fatal dose | Blood level |
Ultra-short acting | 1-2 g | 3 mg/dl |
Short-acting | 2-3 g | 7 mg/dl |
Long acting | 3-5 g | 10 mg/dl |
Trap cases involve forensic techniques to catch individuals red-handed in bribery cases. Phenolphthalein and anthracene are commonly used chemicals in these operations, providing evidence of contact with treated objects (e.g., currency notes).
Phenolphthalein:
Anthracene:
18th Century:
1940s:
Inorganic Insecticides:
Organic Insecticides:
Organochlorines (Chlorinated Hydrocarbons):
Organophosphates:
Carbamates:
Pyrethroids:
DDT (Dichlorodiphenyltrichloroethane) Test:
Organophosphates Test:
Carbamates Test:
Pyrethroid Test:
Gas Chromatography (GC):
High-Performance Liquid Chromatography (HPLC):
Mass Spectrometry (MS):
Infrared Spectroscopy (IR):
Thin Layer Chromatography (TLC):
Nuclear Magnetic Resonance (NMR) Spectroscopy:
UV-Visible Spectrophotometry:
Electron Microscopy (EM):
Environmental Impact:
Health Hazards:
History
|
Controlled Substances Laws classification
Schedule I | high potential for abuse | typically hallucinogens |
Schedule II | equal potential for abuse and medicinal use | generally stimulants |
Schedule III | less potential for abuse and more for medicinal use fall | anabolic steroids and depressant |
Schedule IV | a low potential for abuse and a high potential for medicinal use | typically depressants
2. Barbital 3. Clonazepam 4. Diazepam 5. Phenteramine |
Schedule V | chemicals and precursors typically used in the manufacturing of controlled substances |
Classification of poison (Clinical)
Factors influencing the actions of a poison in the body
|
|
Difference in Drug addiction and drug habituation | ||
Feature | Drug addiction | Drug habituation |
Compulsion | Present | Desire, but no compulsion |
Dependence | Psychological and physical | Psychological, but not physical |
Dose | Tendency to increase | No tendency to increase |
Withdrawal symptoms | Characteristic symptoms | None or mild |
Harm | Both—individual and society | Individual only |
Dependence producing drugs | |||
Drug | Physical dependence | Psychological dependence | Tolerance |
Alcohol | Moderate | Moderate | Mild |
Cannabis | Little | Moderate | Mild |
Cocaine | Little | Moderate | Mild |
Opioids | Severe | Severe | None |
Amphetamine | Moderate | Moderate | Severe |
LSD | None | Mild | Severe |
Barbiturates | Moderate | Moderate | Mild |
Inhalants | Little | Moderate | Severe |
Nicotine | Mild | Moderate | Mild |
Caffeine | Mild | Moderate | Mild |
Poison | Test | Colour |
Nicotine | Roussin’s | Ruby Red needle shaped crystals |
Schindelmeiser | Rose Red | |
Para dimethyl Amino Benzaldehyde | Pink | |
Dragondorf | Yellow | |
Meare | White ppt | |
Silicon Tungustin | Yellow White ppt | |
Phosphomoliptic | Yellow White ppt | |
Datura | Vitali’s | Violet changes to Red |
Barbiturates | Dille – Koppayani | purple or blue violet colour |
Zwikker | Purple to weak blue, for non-thiobarbiturate Green for thiobarbiturate | |
Opium Alkaloids | Frohde’s | violet colour changing to Green |
Marquis | purple red colour changes to violet and finally Blue | |
Husmann’s | reddish-brown or black colour then blood red then to Reddish yellow finally fades | |
Urotropine | Purple -blue-Green | |
Phenol | Ferric chloride | violet to Blue |
Ware’s nitrite – nitrate | ||
Liebermann Nitroso | Green and Blue | |
Cannabis | Fast Blue B Test | purple red |
Duquenois-Levine Test | violet. | |
Cocaine | Scott Test | intense blue |
Gold Chloride Test | rosette or long rod shaped crystal | |
Ethyl Alcohol | Iodoform test | Hexagonal crystals of iodoform |
Sulphomolybdic acid | deep blue | |
Ethyl Benzoate Test | sweet fruity | |
Isopropyl alcohol | Iodoform Test | Pink |
Methanol | Schiff’s reagent Test | Purple colour |
Chromotropic Acid Test | Violet color | |
Chloroform | Fujiwara Test | pink to red |
Chloral hydrate | Fujiwara Test | pink to red |
Nessler’s reagent Test | yellow or reddish brown precipitate changing to grey or black | |
Resorcinol – Potassium bromide | violet and finally to a reddish tint | |
Hydrocyanic acid | Prussian blue Test | a precipitate |
Nitroprusside Test (Vortman’s) | bluish green colour to Yellow | |
Sulphocyanate Test | blood red colour | |
Arsenic | Reinsch’s Test | octahedral crystals of arsenious oxide |
Gutzeit Test | yellow stain | |
Marsh’s Test: (Quantitative) | the octahedral crystal of arsenious trioxide | |
Antimony | Reinsch’s Test | needle shaped crystals of Sb2O3 |
Irritant | Common name | Found in | Active principles | Signs and symptoms | MLA |
Ricinus Communis | (Castor) | wastelands. | Ricin All parts poisonous esp seeds except oil
| The Case of the Umbrella murder Biological warfare /weapon | |
Croton Tiglium | (Jamalgota) | All parts Euphorbiaceae family | Crotin crotonoside, | Accidental poisoning | |
Abrus Precatorius | (Rati, Gunchi, Jequirity | All parts Leguminosae family | Anton Abrine Abralin Hemagglutinin | Fragments of needle(suis) Vilernine snake | cattle poison malingerers abortifacient |
Semecarpus Anacardium | `marking nut ‘bhilawa’ | Semecarpol bhilawanol. | Bruises,brown color urine | criminal abortion,false charge of assault simulate bruises | |
Capsicum Annum | Red pepper or lal mirch | Capsaicin capsicin | |||
Calotropis | (‘Rubber bush’) | Uscharin, calotoxin, calactin, gigantin and calotropin | cattle poison,artificial bruise,snake charmers | ||
Claviceps purpurea | Ergot | abortifacient. |
Toxic component | Colour | Characteristic features | Use |
Arsenious oxide or arsenic trioxide or sankhya, somalkhar, white arsenic or arsenic | White | No taste or smell and is sparingly soluble in water. Most toxic | Weed-killers, insecticides, rat poisons, flypapers |
Copper arsenite | Scheele’s green | ||
Copper acetoarsenite | Paris green or emerald green | Coloring agent | |
Sodium and potassium arsenate | |||
Arsenic sulphide & trisulphide or orpiment(hartal) | Yellow or Orange | ||
Realgar or arsenic disulphide | Red | Coloring pigment and in flypaper | |
Arseniuretted hydrogen or arsine | Colourless | Garlic-like odor |
Sample | Arsenic content Greater than |
Urine | 50 µg/l in 24 hr |
Blood | serum arsenic > 0.9 µg/dl |
Hair | 75 µg% |
Nail | > 100 µg% |
Radiopaque sign on abdominal X-ray | |
Significant ECG | |
Tests | Marsh, Reinsch and Gutzeit tests, Neutron activation analysis & atomic absorption spectroscopy |
if ingested as it is poorly absorbed from the GIT.
Compounds | Uses |
Lead acetate (sugar of lead) | Earlier used as an astringent and local sedative for sprains |
Lead tetroxide (red lead or vermilion) | Used as sindoor |
Tetraethyl lead | Antiknock for petrol |
Lead sulfide | Applied on the eyes |
Lead carbonate (white lead) | Manufacture of paints |
Toxic component | Colour | Characteristic features | Use |
Copper sulphate, Blue vitriol, bluestone, nila tutiya, CuSO4 | Blue crystals | Soluble in water & styptic taste | Fungicide |
Copper subacetate (verdigris) | Blue-green | Coloring agent | |
Copper carbonate | Blue-green | Fungicide |
Toxic Compounds | Colour & Characteristics | Uses |
Thallium acetate | Colorless & tasteless | Fireworks, rodenticide & insecticide |
Thallium sulphate | Rodenticide & insecticide |
OTHER POISONS
Spinal Poisons
Spinal poison:
Peripheral nerve poison:
Other examples:
Differentiation: Strychnine poisoning and tetanus | |||
S.No. | Feature | Strychnine poisoning | Tetanus |
History of injury | None | Present | |
2. | Onset | Sudden | Gradual |
3. | Site of action | Postsynaptic membrane | Presynaptic membrane |
4. | Muscles affected | All muscles affected at the same time | Not affected at the same time |
5. | Lower jaw | Does not start in, nor especially affect the jaw | Starts in and affects the jaw (lock-jaw) |
6. | Muscular condition | Relaxed in between convulsions | Rigid |
7. | Fatal period | 1-2 h | > 24 h |
8. | Chemical analysis | Strychnine found | No poison |
9. | Culture | No growth | Clostridium tetani found |
10. | Progression | Steadily worse/steadily better | Progress rarely steady. Variations and longer remission not uncommon |
Alphos (Aluminum Phosphide)
Features | Bhang | Ganja | Charas |
Other names | Siddi, Patti, Sabji | Marijuana | Hashish |
Source | Dried leaves and fruit shoots | Flowering tops of female plant | Resinous exudates from leaves and stem dried & compressed into blocks |
Colour | Brownish | Rusty green color | Dark green or brown |
Active ingredient % | 15%. (least potent) | 25% ( Mediumly potent) | 25-40% ( Most potent) |
Uses | Beverages, Sweets(Majoon) | Mixed with tobacco & smoked | Mixed with tobacco & smoked |
Sign & Symptoms:
System | Signs and symptoms |
Local | Feeling of numbness or tingling at the place of application. |
Face | Flushed. |
Skin | Pale. |
GIT | Bitter taste, dryness of mouth, vomiting, diarrhea, hyperactive bowel sounds |
CNS | Feeling of well-being, euphoria, restlessness, excitement, talkativeness, delirium, maniacal, hallucinations, tremors (e.g. twitching of small muscles, especially facial and finger) and tonic clonic seizures. Reflexes are exaggerated. Tachypnea, dyspnea, cyanosis |
RS | Tachypnea, dyspnea, cyanosis |
CVS | Tachycardia, hypertension, ventricular arrhythmias. |
Temperature | Hyperthermia. |
Ocular | Pupils are dilated resulting in blurred vision |
System | Signs and symptoms |
CNS | Coma, areflexia, pupils fixed and dilated, flaccid paralysis and loss of vital support functions. |
CVS | Ventricular dysrhythmias result in weak, rapid, irregular pulse and hypotension, circulatory failure and cardiac arrest. |
RS | Cheyne-Stokes respirations, apnea, pulmonary edema, cyanosis, respiratory failure |
Feature | Datura seeds | Capsicum seeds |
Size | Large and thick | Small and thin |
Shape | Kidney-shaped | Rounded |
Color | Dark brown | Pale yellow |
Convex border | Double edge | Single edge |
Smell | Odorless | Pungent. |
Surface | Small depression | Smooth |
Taste | Bitter | Pungent |
Cut section of Seed | Embryo curved outward | Embryo curved inwards |
Asphyxiant gas:
COHb (%) | Signs and symptoms |
0-10 | No symptoms. |
10-20 | Breathlessness, mild headache, abdominal pain. |
20-30 | Throbbing headache, irritability, emotional instability, buzzing in the ears. |
30-40 | Severe headache, nausea, vomiting, dizziness, dimness of vision, confusion, ataxia. |
40-50 | Increasing confusion, hallucinations, rapid respiration, staggering and incoordination— mistaken for drunkenness. |
50-70 | Weak thready pulse, hypotension, irregular respiration, convulsions, coma and death. |
> 80 | Rapid death from respiratory arrest. |
COHb (%) | Severity of poisoning |
10-30 | Mild |
30-40 | Moderate-severe |
> 40 | Very severe |
H2S conc. (ppm) | Clinical effects |
> 200 | Anosmia, pulmonary edema |
> 500 | Hyperpnea, apnea |
> 1000 | Respiratory paralysis, death |
Natural alkaloids
Pink froth from mouth
Purple-red color is observed which gradually changes to violet.
Area | Acute poisoning | Chronic poisoning |
CVS | Tachycardia then bradycardia, tachypnea then respiratory depression & collapse | Anemia , papillations , angina pectoris( chest pain) , Berger’s disease |
GIT | Burning acid sensation , vomiting, abdominal pain , salivation & odor of tobacco | Anorexia , diarrhea , vomiting |
CNS | Headache , restlessness, confusion , vertigo , convulsions coma | Impaired memory , blindness , insomnia |
RS | Cough , wheeze , lung cancer |
Class of Poison | Classical Methods | Modern Methods |
Gases | Micro-diffusion, Adsorption-Desorption | Sensor Based Gas Analyzer, Gas Chromatography |
Volatile Inorganic | Gutzeit Method, Marsh Berzelius Method, Microdiffusion, Digestion with specific reagents / under specific conditions of PH | Microwave oven technique for digestion followed by Ion Chromatography, Spectroscopy(Mass) |
Volatile Organic | Distillation, Steam Distillation, Diffusion | Chromatographic methods |
Non-Volatile Inorganic | Dry and wet ashing, Group analysis, Electrodialysis, Digestion under appropriate analytical conditions, Paper and Thin layer chromatography | Microwave oven technique for digestion followed by Ion Chromatography using Ion-exchange resins |
Non-Volatile Organic | Solvent Extraction, StasOtto, Digestion with ammonium sulphate, sodium tungstate or other modified methods of the above | HPLC, Paired ion extraction Chromatography, HPTLC, Supercritical fluid chromatography, Solid phase extraction, Micellar extraction, Affinity chromatography, Microwave Assisted Reaction System, Accelerated solvent Extraction, Sweep CoDistillation Universal Trace Residue Extraction. |
Anion | Dialysis, Chemical Digestion, Paper and Thin Layer Chromatography | Ion-Chromatography by Ion Exchange resins. |
Cation | Dry Ashing & Wet Digestion |
Poison | Sealing/Liberating Agent | Absorbent/ reactant | Detection |
Carbon Monoxide | 10% Sulphuric acid | Palladium chloride solution (0.5%) in 2N HCl-acts as detection reagent | Palladium Chloride solution turns grey to black |
Ethanol | Saturated sodium carbonate solution | Potassium dichromate solution | orange to green |
The group includes
Extraction of Non-volatile organic poison can be divided into:
Modified Stas-Otto Method:
Summary
Ammonium sulphates method
Wet digestion
Solid Phase Microextraction (SPME):
Solvent Extraction:
A system of two immiscible liquid is required for the separation of material by solvent extraction. The active constituent should be unevenly soluble in the system thereby facilitating extraction of the constituent from one phase to the other. The efficiency of extraction is determined by distribution coefficient (D).
Total wt (gms.) of solute in the organic phase
D = _____________________________________
Total wt (gms.) of solute in the aqueous phase
If one of the two liquids contains a solute, this method is found to be more suitable. The system, in this case is first shaken and then allowed to settle. Some of the solute is transferred to the other liquid. Each of the liquid in a mixture of two immiscible liquids of this kind is referred to as a phase. Thus, some of the solutes is transferred from one phase to the another phase. The amount transferred depends on the relative affinity of the solute for each of the two solvents (relative solubility). It is determined by D. Greater the value of D, greater is the efficiency of extraction.The immiscible system may involve two organic solvents. The extraction for this system may be impaired due to formation of emulsion. Solvent extraction is a common technique in forensic toxicology related to biological matrices. Solvent extraction method has now been upgraded and made automatic viz. accelerated solvent extraction. In case of solid non-biological matrices, continuous extraction by a soxhlet may be employed i.e. continuous extraction.
Cleanup: procedure to remove unwanted impurities that can be in the sample after extraction.
Cleanup can be done by:
Contains:
Ballistics is the scientific study of the motion and behavior of projectiles, particularly bullets, when fired from weapons. It plays a vital role in forensic science, military operations, and criminal investigations. The term “ballistics” originates from the Greek word “ballien,” meaning “to throw.” Originally, it referred to the study of the trajectories of thrown objects, and now it’s specifically used for studying the motion and impact of bullets and other projectiles.
The study of ballistics is categorized into three main branches:
Internal Ballistics:
Focuses on the processes inside the firearm from the moment the trigger is pulled until the bullet exits the barrel.
External Ballistics:
Examines the behavior of the bullet as it travels through the air after leaving the firearm.
Terminal Ballistics:
Investigates the effects of the bullet upon impact with the target, including penetration, fragmentation, and energy transfer.
Internal ballistics deals with what happens inside the firearm after the trigger is pulled. This includes:
Ignition of the Propellant:
The firing pin strikes the primer, igniting the gunpowder or propellant.
Pressure Build-Up:
The burning propellant generates gases, causing high pressure within the cartridge.
Projectile Movement:
The pressure propels the bullet down the barrel, rapidly accelerating it.
Barrel Interaction:
The bullet interacts with the rifling (spiral grooves inside the barrel), which imparts a spin that stabilizes the bullet’s flight.
External ballistics focuses on the bullet’s flight once it exits the firearm. Several factors influence this phase:
Gravity:
Gravity causes the bullet to drop as it travels.
Air Resistance:
Air resistance slows the bullet and affects its trajectory.
Wind:
Wind can alter the bullet’s path, making accurate shooting more difficult.
Spin Stabilization:
The spin from the rifling stabilizes the bullet, helping it maintain a straighter trajectory.
Terminal ballistics studies the bullet’s interaction with the target. This phase is crucial for assessing the effectiveness of a bullet in various contexts, including hunting, military, and forensic applications. Key aspects include:
Penetration:
How deep the bullet penetrates the target.
Wound Cavity:
The path created by the bullet as it passes through the target.
Fragmentation:
Some bullets are designed to fragment upon impact, causing additional damage.
Energy Transfer:
The amount of kinetic energy transferred to the target, affecting the stopping power.
The ballistic coefficient (BC) measures a bullet’s ability to overcome air resistance. A higher BC indicates better aerodynamic efficiency, meaning the bullet retains its velocity and is less affected by wind and gravity. Projectile design is crucial in determining BC:
Shape:
Pointed or boat-tail designs reduce air resistance, increasing the BC.
Mass:
Heavier bullets generally have higher BCs, as they retain momentum better.
Material:
The material affects the bullet’s density and hardness, influencing its ballistic performance.
Forensic ballistics applies ballistics in criminal investigations, involving the examination of firearms, ammunition, and their effects.
Legal Definition of Firearms
In legal terms, The Indian Arms Act, 1959 provides the legal definition for firearms, encompassing everything from manufacturing to usage.
This definition also covers parts of firearms, silencers, and equipment designed to control or modify the use of firearms.
Key areas of Forensic Ballistics include:
Firearm Identification:
Linking a bullet or cartridge case to a specific firearm based on unique markings.
Trajectory Analysis:
Reconstructing a bullet’s path to understand a shooting incident.
Gunshot Residue (GSR) Analysis:
Detecting residue on a suspect’s hands or clothing to confirm if they fired a weapon.
Wound Ballistics:
Analyzing injuries caused by bullets to determine factors like weapon type, shooting distance, and trajectory.
Ballistics plays a vital role in crime scene investigations involving firearms. Forensic experts use ballistic evidence to:
Reconstruct Shooting Incidents:
By analyzing trajectories and positions, investigators can recreate the events.
Identify Weapons:
Unique markings on bullets and cartridge cases help link specific firearms to crimes.
Estimate Range of Fire:
The distance between the shooter and the target can be determined through ballistic evidence.
Recent technological advancements have greatly improved firearm accuracy, range, and effectiveness. Key developments include:
Precision-Guided Munitions (PGMs):
Munitions designed to hit targets with high precision using GPS or laser guidance.
Advanced Materials:
The use of materials like tungsten and depleted uranium increases bullet penetration and effectiveness.
Smart Ammunition:
Ammunition that can adjust its trajectory mid-flight or detonate at a specific point.
Computerized Ballistic Calculators:
Devices that help shooters account for various factors to make accurate long-range shots.
Several key personalities have significantly contributed to forensic ballistics:
This definition includes:
Pump-Action Shotgun:
Break-Action Shotgun:
Semi-Automatic Shotgun:
Firing Pin Impressions:
Extractor and Ejector Marks:
Breech Face Marks:
Headstamp Analysis:
Tools and Techniques in Firearm Forensics
Velocity is the speed at which a projectile (bullet, shot, or slug) travels after being fired from a firearm. It is typically measured in feet per second (fps) or meters per second (m/s).
Penetration refers to the projectile’s ability to pass through a target, including tissue, barriers, or other materials.
Dispersion is the spread of projectiles as they travel, particularly in shotguns firing pellets or multiple projectiles.
Ricochet occurs when a projectile strikes a surface and deflects, changing its trajectory.
An accidental discharge occurs when a firearm is unintentionally fired, often due to negligence, mechanical failure, or improper handling.
Determination of range involves estimating the distance between the firearm and the target at the time of discharge.
Gunshot Residue (GSR) Patterns:
Spread of Shot (Shotguns):
Wound Characteristics:
Projectile Penetration:
Visual Inspection:
Photographic Documentation:
Serial Number Examination:
Operational Testing:
Safety Checks:
Controlled Environment:
Ammunition Assessment:
Projectile Examination:
Shell Casing Analysis:
Trajectory and Range Determination:
Gunshot Residue (GSR):
Material Composition:
Association with Ballistic Evidence:
Fingerprint and DNA Analysis:
Definition: The study of projectile motion within the firearm, from the initiation of the primer to the moment the projectile exits the barrel (muzzle).
Firing Sequence:
Bullet Travel through the Barrel:
Definition: The study of projectile motion in flight, from the moment it exits the muzzle to when it reaches the target.
Projectile Trajectory:
Forces Acting on the Projectile:
Velocity Types:
Environmental Influences:
Definition: The study of the effects of a projectile upon impacting a target, including penetration, fragmentation, and energy transfer.
| Aspect | Internal Ballistics | External Ballistics | Terminal Ballistics |
|---|---|---|---|
| Focus | Motion within the firearm | Motion in the air | Motion upon impact |
| Key Factors | Chamber pressure, barrel length, rifling | Gravity, air resistance, spin stability | Penetration, cavitation, energy transfer |
| Applications | Firearm efficiency, tool mark analysis | Trajectory and range determination | Wound analysis, target material behavior |
Tool marks are categorized as class evidence or individual evidence, depending on their characteristics:
Documentation:
Casting:
Tool Recovery:
Comparison Microscopy:
Test Marks:
Measurements and Profiling:
Material Transfer Analysis:
C. Factors in Tool Mark Analysis
Condition of the Tool:
Material of the Marked Surface:
Tool Angle and Pressure:
Restoration of erased markings, such as serial numbers on firearms, tools, or machinery, is a crucial forensic process. Serial numbers or other identifying marks are often obliterated to conceal an item’s origin. Forensic experts can often recover these marks using scientific techniques.
Visual Examination:
Magnetic Particle Inspection (For Ferromagnetic Metals):
Ultrasonic Testing:
Acid Etching:
Procedure:
Reagents:
Procedure:
Reagents:
Procedure:
Reagents:
Procedure:
Reagents:
Procedure:
6. Zinc Alloys
Reagents:
Procedure:
Polishing:
Microscopic Analysis:
X-Ray Imaging:
Scanning Electron Microscopy (SEM):
Laser Scanning:
Fire: A rapid oxidation process that produces heat, light, and by products such as gases and smoke.
Arson: The deliberate act of setting fire to property with criminal intent.
Combustion Process:
Fire Behavior:
Combustion Triangle:
Flash Point and Fire Point:
Types of Flames:
Fire patterns or fire indicators are physical evidence left after a fire that provide insight into the fire’s origin, cause, and progression. They include observable effects such as charring, distortion, oxidation, melting, color changes, and structural collapse. These patterns help forensic fire investigators identify critical details about the fire dynamics.
The “V” pattern is a key fire indicator caused by the upward and outward movement of heat and flames.
Indoor Fires:
Outdoor Fires:
Lines of demarcation are visible borders or patterns that separate heat-affected regions from adjacent unaffected or less affected areas.
These patterns help identify the direction of fire spread and reveal regions exposed to high or low heat intensity. They are often used to trace the location of the fire’s origin. Darkened areas (smoke-stained) stand in contrast to lighter, clean areas, which experienced less heat exposure.
Low burns occur when the fire burns downward and creates a low-level burn pattern. These patterns are often observed near the fire’s point of origin because fires typically burn upward and outward but can also burn downward when accelerants are used.
Penetrations are burn-through holes in floors or surfaces, usually caused by intense heat or the use of accelerants. These patterns help locate origin and measure fire intensity.
Charring refers to the carbonization or partial burning of materials, commonly observed in wood.
Charring is used to evaluate fire intensity, duration of exposure, and spread direction. The depth of charring generally reduces with distance from the fire’s origin.
Clean burn occurs when a surface is exposed to direct flame impingement, leaving a clean, heat-affected area.
Surfaces appear clean due to the absence of soot and residues. Clean burn patterns indicate intense, localized flames, often revealing fire hotspots and fuel sources. They are critical for determining fire movement and spread.
A trailer refers to a line or trail of combustible material (e.g., paper, clothing, ignitable liquids) intentionally placed to spread fire from one location to another.
Pour patterns are irregular burn patterns on the ground or surfaces caused by the deliberate pouring of an ignitable liquid. These patterns appear as irregular-shaped or puddled burns. Deep charring and unusual burn intensities often suggest the use of accelerants.
These patterns indicate the deliberate setting of fires and are critical in identifying cases of arson.
Rollover occurs when unburned combustible gases that accumulate near the ceiling ignite suddenly, causing flames to roll across the ceiling surface.
This phenomenon happens in the incipient stage of a fire and signals a transition to flashover. Rollover is an important indicator of high temperatures and incomplete combustion of fuels.
Flashover is a sudden, simultaneous ignition of all combustible materials in a room or compartment due to extreme heat.
It occurs when the temperature of materials rises above their ignition points. Flames spread rapidly over surfaces, windows may shatter from thermal stress, and the entire compartment becomes engulfed. Flashover marks the full involvement phase of a fire, often leading to structural collapse and severe damage.
Backdraft is an explosive event caused by the sudden reintroduction of oxygen into an oxygen-depleted, smoldering fire.
Fires in enclosed spaces with limited ventilation produce combustible gases. When an opening introduces oxygen, the gases ignite explosively.
Indicators of backdraft include:
Backdraft is extremely dangerous and provides critical clues to fire progression and ventilation conditions during investigations.
The transfer of heat energy is a fundamental process in fire dynamics. Heat moves through three primary mechanisms: Radiation, Convection, and Conduction. Understanding these mechanisms helps explain the fire’s behavior, spread, and effects on surrounding materials.
Radiation is the transfer of heat energy through electromagnetic waves emitted by a body due to its temperature.
Significance in Fire Dynamics:
Radiation allows heat transfer over distances, enabling flames to ignite nearby combustible materials, even without direct contact. It plays a significant role in fire spread within enclosed spaces.
Convection is the transfer of heat through the movement of fluids (liquids or gases) caused by differences in temperature and density.
Significance in Fire Dynamics:
Convection is the primary mechanism for heat transfer in fire plumes, smoke movement, and the spread of fire through open spaces. Hot smoke and gases rise, accumulating at high levels, which can lead to flashover.
Conduction is the transfer of heat through direct contact within or between materials. It occurs when heat energy flows from a region of high temperature to a region of low temperature within a solid medium.
Conduction is categorized based on material thickness into:
Significance in Fire Dynamics:
1. Incipient/Initial/Growth Phase
2. Free-Burning Phase (Fully Developed Stage)
3. Smoldering Phase (Decay Stage)
Fires are classified based on the type of fuel involved. Each class requires specific methods and extinguishing agents to effectively control and suppress the fire.
The examination of a fire scene is crucial for identifying its cause and gathering evidence.
Extraction of Exhibits/Samples
Gas Chromatography (GC):
Detector: Flame Ionization Detector (FID)
Carrier Gas: Nitrogen or Helium
Flow Rate: 30 mL/min
Temperature Settings:
Mass Spectrometry (MS):
Headspace Analysis:
Fourier Transform Infrared Spectroscopy (FTIR):
Thin-Layer Chromatography (TLC):
Capillary Gas Chromatography
Solid Fuels:
Liquid Fuels:
Gaseous Fuels:
Combination Materials:
Visual Indicators:
Portable Detection Devices:
Canine Detection:
Origin and Cause Determination:
Matching Accelerants:
Fire Suppression Interference:
Low or Deflagrating Explosives
High or Detonating Explosives
| Explosive | Detonation Speed (m/s) |
|---|---|
| Amatol | 6430 |
| Tetryl | 7850 |
| RDX | 8130 |
| TNT | 8250 |
| PETN | 8300 |
Primary Explosives
Secondary Explosives
Dynamite
ANFO (Ammonium Nitrate Fuel Oil)
Slurry Explosives
Water Gel Explosives
Explosive Emulsions
| Name | Composition |
|---|---|
| A-3 | RDX + Wax |
| B | RDX + TNT + Wax |
| C-1 | RDX + TNT |
| Cyclotol | RDX + TNT + Tetryl |
| PTX-1 | RDX + TNT + PETN |
| PTX-2 | PETN + TNT |
| SEMTEX | RDX + PETN |
| Pentilite | PETN + TNT |
| Terytol | TNT + Tetryl |
| Tritnoll | TNT + AL (Aluminium) |
| Picratol | TNT + Ammonium Picrate |
| Octol | HMX + TNT |
| Delta Sheet | PETN + Plasticizer |
| Amonal | TNT + Ammonium Nitrate + AL + Carbon |
| Amatal | TNT + Ammonium Nitrate |
Pyrotechnics are used for producing specific effects like light, sound, and color in fireworks and signaling devices.
Propellants include explosive materials that produce controlled combustion to propel objects, such as in rockets or firearms.
Aerosol Mixtures: Used in propellant systems for generating thrust in jet propulsion and other applications.
Low Explosives:
High Explosives:
Country-made bombs often rely on low explosives, which deflagrate rather than detonate. Common chemicals include:
Black Powder (Gunpowder):
Ammonium Nitrate-based Mixtures:
Fireworks Extracts:
Other Materials:
IED triggers can be highly sophisticated or rudimentary. Types include:
| Type | Description |
|---|---|
| Time-controlled | Explodes after a delay using clocks, timers, or programmable circuits. |
| Command-controlled | Detonated remotely (radio signals, mobile phones). |
| Victim-operated | Triggered by the victim unknowingly (pressure plates, tripwires). |
| Vehicle-borne | Placed in vehicles (cars, trucks) for large-scale destruction. |
| Suicide IEDs | Carried or worn by individuals (e.g., suicide bombers). |
Mechanical Explosions:
Chemical Explosions:
Nuclear Explosions:
Steps in Explosion Investigation
Fire Pattern Analysis:
Temperature Indicators:
Accelerant Indicators:
Techniques for Accelerant Detection
Headspace Analysis:
Char Analysis:
Extraction Methods:
Ion Mobility Spectrometry (IMS):
Fourier Transform Infrared Spectroscopy (FTIR):
Forensic Photography is a systematic method of preserving and documenting evidence for legal proceedings.
Preserving Evidence
Legal Validity
Clarity in Analysis
Wide Angle or Fisheye Lens
Telephoto Lens
Macro Lens
| Type of Photograph | Minimum Number Required | Purpose |
|---|---|---|
| Photo Identifier | 1 photo | Identifies the case, scene, and investigator. |
| Overview Photographs | 4 photos | Provides a general view of the scene. |
| Mid-range Photographs | 4 photos | Shows evidence in context with surroundings. |
| Close-up Photographs | 4 photos per evidence | Captures detailed views of evidence. |
Rule of Thirds
Leading Lines
Framing
Forensic photography encompasses various specialized techniques used to capture critical details. These types include:
To ensure admissibility and accuracy, forensic photography must follow these principles:
Types of Hair
Structure of Hair
Hair comprises three main layers:
Structure of hair
Features of hair
Length | Indefinite |
Diameter | 20-100 micrometre |
Colour | White ,brown, black, grey |
Tip |
|
Roots |
|
Medula | Continuous or discontinuous, fragmentary ,absent |
Cortex | Healthy ,damaged, brittle, fibrous ,air bubbles present or absent |
Pigment |
|
Cuticle |
|
Scales | Prominent ,smooth ,serrated, flattened |
Relative density | 1.3 – 1.4 |
Parts of hair
Cuticle
Cortex
Medulla
Medullary index= diameter of medulla / diameter of the shaft
Root
The three phases of hair
Hair examination
Features | Human | Animal |
Colour | Relatively consistent along shaft | Often showing profound colour changes and banding |
Cortex | Occupying most of the width of shaft greater than medulla | Usually less than width of medulla |
Distribution of pigment | Even, slightly more towards cuticle | Central or denser towards medulla |
Medulla | Less than one-third width of shaft. Amorphous, mostly not continuous when present | Greater than one-third width of shaft. Continuous, often varying in appearance along shaft, defined structure |
Scales | Imbricate, similar along shaft from root to tip | Often showing variation in structure along shaft from root to tip |
Race | Diameter(um) | Color | Cross-section | Orientation | Pigmentation | Cuticle | Undulation |
Negroid | 60-90 | Dense black | Flat | Compressed or flat | Dense & clumped | Prevalent | |
Caucasoid | 70-100 | Dense black in clusters | Oval | Curly,wavy | Evenly distributed | Medium | Uncommon |
Mongoloid | 90-120 | Pale blonde to brown | Round | Rough, straight | Dense auburn | Thick or denser | Never |
Features | Male | Female |
Scap hair | Short and coarse | Long and fine |
Eyebrows | Coarse and thick | Fine and thin |
Hair on pinna | Present | Absent |
Body hair | Good growth | No significant growth |
Pubic hair | Thicker,coarse ,extended upward towards naval | Horizontal, covering only pubis, triangular distribution |
Medullary index | Lower | Higher |
Length (cm) | Shaft | Root | Tip | Confi | Medulla | |
Eye/eyelashes | 5 | Coarse | – | Tapered | Smooth,curved | Large |
Beard | Upto 100 | Irregular | Large | – | Wavy | Continuous (broadest) |
Armpit | Upto 5 | Lighter color,coarse | Large | Frayed | Kinky | Continuous |
Arm/legs | Upto 2 | Lighter color | Small | Curled | Fine | Irregular |
Scalp(head) | Upto 100 | Uniform thickness | Small | Tapered | Wavy | Continuous, discontinuous, fragmented |
Chest | Upto 5 | Variable diameter | Large | Arched | Wavy | Continuous |
Pubic | Upto 6 | Coarse follic | – | Tapered | Complex | Broad |
Analysis of hair
Collection
Examination
Important terms
Examination of Hair in Forensic Science
Applications in Forensic Investigations
Fibers are classified into two major categories based on their origin:
Natural fibers are divided into three categories:
A. Vegetable Fibers
Artificial Fibers from Natural Polymers:
Natural Fibers Synthetic Fibers
Derived from natural sources. Made by humans via chemical processes.
Burns to ash; smells like burnt hair/paper. Melts; emits chemical smell when burned.
Biodegradable;
environmentally friendly. Non-biodegradable;
harmful to the environment.
Good absorbents; retain heat, cold, or sweat. Poor absorbents; lack thermal adaptability.
Length fixed by nature; crimp naturally present. Length customizable; crimp added artificially.
Requires bleaching. No bleaching required.
Less durable; limited applications. Highly durable; versatile in use.
Consists of
Layers of Soil/Soil Profile
Humus: component of soil, formed by the decomposition of plant or animal matter by microorganisms
Soil | Contents |
Alluvial | Potash and lime |
Black | Iron, potassium, magnesium, calcium, Titaniferous Magnetite |
Red | Iron & potash |
Laterite | Iron & potash |
Mountain | Iron & humus |
Arid | Nitrates & phosphorus |
Saline & Alkaline | Salts like sodium, magnesium & calcium |
Peaty & Marshy | Humus, soluble salts & organic matter |
Soil composition
Collection and packing
Examination
Bromoform (ml) | Bromobenzene (ml) | Density (g/ml) |
10 | 00 | 2.890 |
10 | 0.69 | 2.800 |
10 | 1.58 | 2.700 |
10 | 2.63 | 2.600 |
10 | 3.90 | 2.500 |
10 | 7.36 | 2.300 |
00 | 10.00 | 1.499 |
Munsell colour Test
H-hue
V-value
C-chroma
Paint, lacquer, and varnishes are widely used in construction, art, transportation, and industrial processes. In forensic science, they serve as trace evidence, often found at crime scenes involving vehicular accidents, burglaries, or vandalism. Their complex and unique compositions allow forensic experts to link evidence to specific sources or events.
Paints consist of four main components:
Forensic examination involves physical, chemical, and instrumental analysis.
Network forming oxides [Formers] | SiO2 , B2O3 , GeO3 , As2O3 , P2O5 , V2O3 |
Network losing oxides [Modifiers] | Li2O , Na2O , K2O , CaO , MgO , BaO |
Stabilising oxides [Decrease process of hydrolysis] | CaO , MgO, BaO |
Formers/Modifiers [Intermediate Oxides] | Al2O3 , PbO , Sb2O3 , ZnO , TiO2 , BeO |
Optical Glass | SiO2 + K2O + PbO |
Pyrex Glass | Na2B4O7 / Al2O3 + SiO2 |
Window Glass | SiO2 + Na2O + CaO + BaO + Al2O3 + MgO |
Bottle Glass | SiO2 + Na2O + CaO + BaO + Al2O3 |
Plate Glass | Harder than common glass |
Safety Glass(Laminated) | Less radial & more concentric fractures |
Safety Glass(Tampered) | Breaks into rectangular pieces.No radial or concentric fracture |
Glass | Main Component | Use/ Property |
Soda Lime Glass | SiO2 | Used in windows/Bottles |
Borosilicate Glass | B2O3 | Temp. & Acid resistant |
Aluminosilicate Glass | Al2O3 | More temp. Resistant than Borosilicate |
Lead Alkali Silicate Glass | Used in video tube, neon light tube etc., | |
Silica Glass | Chemical, Electric, Temp. resistant | |
Flint Glass |
Element | Effect |
Arsenic | Decolourisation |
Titanium | Air Bubble removal |
Chromium | Green Colour |
Cobalt | Blue Colour |
Sulphur+Iron | Brown Colour |
Liquid | RI | Glass | RI |
Water | 1.333 | Vitreous Silica | 1.458 |
Olive oil | 1.467 | Headlight | 1.47-1.49 |
Glycerin | 1.467 | Window | 1.51-1.52 |
Castor oil | 1.82 | Bottle | 1.51-1.52 |
Clove oil | 1.543 | Optical | 1.52-1.53 |
Bromobenzene | 1.560 | Quartz | 1.54 |
Bromoform | 1.597 | Lead | 1.56-1.61 |
Cinnamon oil | 1.619 | Diamond | 2.419 |
3R rule:
Radial cracks give rib marks that make
Right angles on the
Reverse side from where the force was applied
Examination
(matrix= graphite powder+lithium fluoride + CuO)
Then burnt by using graphite DC arc which gives radiation & recorded
Lime | CaO | 60-67% |
Silica | SiO2 | 17-25% |
Alumina | Al2O3 | 03-08% |
Iron Oxide | Fe2O3 | 0.5-06% |
Magnesia | MgO | 0.1-04% |
Soda & Potash | Na2O & K2O | 0.2-01% |
Sulfur Trioxide | SO3 | 01-2.75% |
Free Lime | CaO | 00-01% |
The Main Constituents of Cement are:
Dicalcium Silicate | 2CaOSiO2 | 30% |
Tricalcium Silicate | 3CaOSiO2 | 40% |
Tricalcium Aluminate | 3 CaOAl2O3 | 11% |
Tetracalcium Aluminoferrite | 4CaOAl2O3 | 11% |
Computer forensics, also known as digital forensics, involves the identification, preservation, analysis, and presentation of electronic evidence for legal purposes. With the rise of technology, computer forensics has become a vital field in solving cybercrimes, corporate fraud, and even traditional crimes that involve digital evidence.
Computer forensics is the branch of forensic science that deals with the recovery, investigation, and analysis of data from digital devices.
It ensures the integrity of digital evidence while maintaining its admissibility in court.
Computer crimes, also referred to as cybercrimes, are illegal activities that involve digital devices or computer networks. These can be broadly categorized as:
Digital evidence is any data stored or transmitted in a digital format that can be used in court proceedings. Examples include:
Acquisition is the process of creating a forensic copy of digital evidence to preserve its integrity.
Forensic examination involves analyzing digital evidence to extract meaningful information relevant to an investigation.
Mobile phone forensics is a branch of digital forensics dedicated to the recovery, preservation, and analysis of data stored on mobile devices. With the increasing reliance on smartphones for communication, banking, and location services, they have become vital sources of evidence in forensic investigations.
Mobile phone forensics involves the extraction, preservation, and analysis of data from mobile devices, including smartphones, feature phones, tablets, and wearable devices.
Fingerprint Types
Patent Prints
Latent Prints
Plastic Prints
Level of Details | Specification |
Level 1 | Class Characteristics(Whorl, Loop, Arch, Composite) |
Level 2 | Minutia (Galton’s Details) |
Level 3 | Edgeoscopy – Small shapes on an individual ridge. Indicated by the green line. Poroscopy – The thickness/thinness of a particular ridge (red line) and the location of sweat pores (blue dots). Sweat pores may not be visible in some prints. |
Embryonic Development | Gestational Period |
Volar Pads form | 6-8 weeks |
Hands Webbed | 8-10 week |
Volar Pads begin to Recede | 10-12 weeks |
Skin Ridge appear | 13 week |
Fingerprint Pattern Completes | 21 week |
Gland formation on the palms and soles | 3 month |
Fingernails reach tip of finger | 8 month |
Epidermis(Stratified Epithelium) | Stratum corneum (cornified layer) |
Stratum lucidum (clear layer) | |
Stratum granulosum (granular layer) | |
Combined stratum spinosum and stratum germinativum Stratum Mucosum (“Marcello” Malpighian layer) | |
Stratum spinosum (prickle cell layer) | |
Stratum germinativum (basal cell layer) | |
Dermis(fibroelastic connective tissue) | Papillary dermis (pars papillaris) |
Reticular dermis (pars reticularis) | |
Subcutaneous fatty tissue |
Latent fingerprint residue is primarily composed of substances secreted by the skin and can be categorized into three main types:
Sebaceous secretions are oily and waxy substances produced by sebaceous glands, mainly found on the face, scalp, and upper body. These secretions can transfer to the fingertips through contact with these areas.
Sebum Composition |
|
The main components include:
Eccrine glands, distributed across most of the body’s surface (including the fingertips), secrete a primarily water-based substance. Key components include:
Composition of Eccrine Sweat | |
Inorganic (major)
| Inorganic (trace)
|
Organic (general)
| Organic (lipids)
Miscellaneous
|
These are external materials transferred to the fingertips and subsequently left on surfaces. Examples include:
The Henry Ten Digit Fingerprint Classification System revolutionized forensic science by introducing a methodical way to classify and store fingerprints, making criminal identification more efficient. Developed by Sir Edward Henry and first implemented at Scotland Yard in 1901, this system laid the groundwork for modern fingerprint databases, including the expansive system used by the FBI.
The Battley Single Digit Classification System, introduced in 1929, was a groundbreaking advancement in fingerprint analysis, particularly for cases involving partial prints. Developed by Chief Inspector Harry Battley and Detective Superintendent Fredrick Cherrill of New Scotland Yard, this system transformed the way forensic investigators identified suspects using incomplete fingerprint evidence.
Origins: Developed in 1929 by Harry Battley and Fredrick Cherrill at New Scotland Yard.
Necessity: Aimed to address the challenge of identifying suspects from partial fingerprints, which are common at crime scenes.
Impact: Streamlined the identification process, making it faster and more efficient.
Key Features: Each card recorded a single fingerprint along with:
Criminal Reference Number: For tracking the individual.
Henry Classification: Incorporated if additional classification was needed.
Unique Identifiers: Specific details to differentiate each fingerprint.
Pattern Analysis: Fingerprints were divided into arches, loops, and whorls.
Ridge Counts & Deltas: Detailed examination of ridge counts and delta positions enhanced classification accuracy.
Innovative Tool: A reticle-based magnifying glass used for precise measurement.
Functionality:
Measured distances between key features like the core and delta.
Classified fingerprints based on their position within concentric circles.
Unique Code: Each fingerprint was assigned a unique alphabetical code based on its features.
Detailed Classification: The system generated more detailed data than previous methods, such as the Henry classification.
Efficient Identification: Allowed forensic teams to quickly match partial prints to suspects.
Data Management: Although effective, the sheer volume of data eventually posed challenges in handling and storage.
Alternative Methods: Other single-digit classification systems included:
Collins System
Larson System
Stockis System
Sagredo System
Oloriz System
Distinctiveness: Battley’s system was distinguished by its precision and the innovative circle-reading subdivision.
The process begins with locating fingerprints at a crime scene. Fingerprints can be broadly categorized into three types: visible, plastic, and latent.
Visual Inspection: The initial step involves a keen visual examination of surfaces for visible fingerprints, often found on smooth, non-porous materials like glass, metal, or plastic. These prints are usually left behind by substances such as dirt, grease, or blood.
Alternate Light Sources (ALS): When fingerprints are not visible to the naked eye, forensic experts utilize alternate light sources, such as UV light. These specialized lights cause latent fingerprints to fluoresce, revealing patterns that were previously invisible. ALS can highlight fingerprints on diverse surfaces, from walls to fabrics, making them a versatile tool in forensic investigations.
Once potential fingerprint locations are identified, various techniques are employed to make these prints visible. The choice of method depends on the type of surface and the nature of the print.
Powder Methods: Specially formulated powders, available in various colors and compositions, are gently dusted over surfaces to reveal prints. These powders adhere to the oils and sweat residues left by fingerprints. This method is particularly effective on smooth, non-porous surfaces like glass, metal, and polished wood.
Fuming Methods: Cyanoacrylate Fuming (Superglue Method): Ideal for non-porous surfaces, this method involves heating superglue in a fuming chamber. The resulting vapors adhere to the fingerprint residues, forming a white polymer that outlines the print. Iodine Fuming: Best suited for porous surfaces such as paper, iodine fumes react with the natural oils in fingerprints, creating a temporary brownish print that can be photographed.
Chemical Methods: Ninhydrin: Used on porous surfaces like paper or cardboard, ninhydrin reacts with amino acids in fingerprint residues, producing a distinctive purple color. This reaction helps visualize prints that are otherwise undetectable by powder or fuming methods.
After making fingerprints visible, the next critical step is their collection and preservation.
Lifting Techniques: Adhesive Lifting Tape: A clear, adhesive tape is pressed firmly over the developed print. When the tape is peeled off, it lifts the fingerprint from the surface. The tape is then placed on a contrasting background, such as a black or white backing card, to enhance visibility and protect the print.
Gel Lifters: These are used on uneven or textured surfaces where traditional tape might not be effective. Gel lifters conform to the surface, ensuring the complete transfer of the print.
Packaging: Collected fingerprints are carefully packaged to prevent contamination or physical damage. They are placed in protective covers, labeled accurately, and stored in a controlled environment.
Preserving fingerprint evidence is crucial to maintaining its integrity for analysis and potential courtroom presentation.
Storage Conditions: Fingerprint evidence is stored in a dry, secure location. Moisture and physical handling are avoided to prevent deterioration or accidental smudging.
Chain of Custody: Every individual who handles the fingerprint evidence must be documented. This ensures the evidence’s traceability from the crime scene to the courtroom, safeguarding its credibility in legal proceedings.
The final stage involves a thorough examination of the collected fingerprints to extract actionable insights.
Detailed Analysis: Examining Ridge Patterns: Forensic experts meticulously analyze ridge patterns, minutiae points (such as bifurcations and ridge endings), and other unique characteristics. Comprehensive Reports: A detailed report is generated, documenting the methods used for detection, the condition and location of the prints, and the findings from the analysis.
Laboratory Examination: If further analysis is required, the fingerprints are sent to specialized forensic laboratories. Here, advanced techniques such as digital enhancement or comparison with existing databases (like AFIS – Automated Fingerprint Identification System) are employed.
Particle Size and Shape:
Alternative Application Methods:
Different types of powders cater to various surfaces and conditions:
Composition of Powders:
The Physical Developer (PD) Method is a fingerprint detection technique designed for porous surfaces, such as paper, rubber gloves, raw wood, and adhesive tapes. This method is particularly effective in challenging conditions where other techniques fail, such as wet or water-damaged materials. PD involves a chemical process similar to photographic film development, allowing the visualization of fingerprints even on substrates exposed to moisture.
Principle of the Physical Developer Method
The PD method works by utilizing a redox reaction involving Silver Nitrate (AgNO₃). When a latent fingerprint comes into contact with the PD solution, silver ions in the solution are reduced to metallic silver, which adheres to the lipids and fatty acids in the fingerprint residue, revealing the print as a dark gray or black image. The presence of a detergent in the solution prevents silver particles from clumping, maintaining the clarity and structure of the fingerprint through a process called peptization.
Components of the Physical Developer Solution
The PD solution is made up of four main components:
Chemical Process Behind the PD Method
Step-by-Step Procedure for the Physical Developer Method
Applications
The PD method is widely used on surfaces where water exposure would typically eliminate traces of amino acids:
Advantages and Limitations
The Silver Nitrate Method is a classic forensic technique used for developing latent fingerprints, particularly effective on porous surfaces such as paper, cardboard, and untreated wood. This method reveals fingerprints by leveraging a reaction between chloride ions present in sweat and silver nitrate, producing dark ridges that stand out on the evidence surface.
Mechanism
Procedure for Using the Silver Nitrate Method
Pros and Cons of the Silver Nitrate Method
Applications and Limitations
The Ninhydrin Method is a chemical process used to reveal latent fingerprints on porous surfaces, such as paper and wood, by reacting with amino acids in the fingerprint residue. This technique can develop fingerprints that have been undetectable for years, making it an invaluable tool in forensic investigations, especially for old or archival evidence.
Understanding Latent Fingerprints
Ninhydrin is a chemical that reacts with amino acids in fingerprints to produce a purple compound called Ruhemann’s Purple, making the fingerprint visible. Ninhydrin is particularly effective on porous materials like paper, cardboard, and wood.
Procedure for Using Ninhydrin
Enhancing Fingerprints
For sharper prints or to enhance faded prints, post-treatment can be done using metal salts such as zinc chloride. Under specific lighting, such as arc or xenon lamps, these treated prints can fluoresce, which helps in improving contrast and clarity.
Ninhydrin Analogs
Sometimes, standard ninhydrin may not work effectively on certain surfaces. In such cases, modified forms of ninhydrin are used:
Advanced Techniques
Applications and Limitations
The DFO Method (1,8-Diazafluoren-9-one) is a powerful and advanced technique used for detecting latent fingerprints, especially on porous surfaces like paper, cardboard, and wood. What sets DFO apart from other methods is its ability to reveal fingerprints that glow under specific lighting conditions, making it a go-to method for investigators when clarity and speed are critical.
What Makes Latent Fingerprints Visible?
Latent fingerprints are formed when sweat from our fingertips leaves behind invisible marks on surfaces. These marks contain various substances, including amino acids, which are essential for fingerprint detection. The DFO method works by reacting with these amino acids to reveal the prints.
How the DFO Method Works
Advantages of the DFO Method
Limitations of the DFO Method
The Fluorogenic Method is a powerful forensic technique that leverages fluorescent chemicals to reveal latent fingerprints. These reagents react with amino acids and other compounds in sweat residue to produce bright, colorful, and sometimes glowing prints, making them ideal for detecting fingerprints on porous surfaces such as paper, wood, and cardboard.
Key Fluorogenic Reagents
Each fluorogenic reagent has unique properties, enabling forensic experts to select the most suitable one depending on the type of evidence and desired visualization effect.
Advantages of the Fluorogenic Method
Quick Visualization: Often faster and more efficient than other fingerprint detection methods.
The Iodine Fuming Method is an effective tool in forensic science for revealing latent fingerprints, especially on porous surfaces like paper, wood, and fabric.
The Iodine Fuming Method involves heating iodine crystals, which turn into violet-colored vapors. These vapors interact with the oils and fats left behind in fingerprint residue, revealing the ridge patterns of the print in a yellowish-brown hue. This method works particularly well on old prints or materials that other techniques might not effectively treat.
The Science Behind the Method
Iodine doesn’t chemically bond to the fingerprint residue. Instead, it physically absorbs onto the fatty acids left behind by sweat, causing a color change that makes the ridge details visible. However, there’s a catch: Iodine-developed prints fade quickly in the air, so it’s crucial to act fast!
How to Apply the Iodine Fuming Method
There are three primary ways to apply the iodine fuming technique:
Making the Iodine Prints Permanent
Since iodine-developed prints fade quickly, there are methods to make them permanent for further analysis:
Pros and Cons of the Iodine Fuming Method
Pros:
Cons:
Toxicity: Iodine vapors are toxic, so safety precautions are necessary during the process.
Phase Transfer Method is cost-effective, revealing latent fingerprints on almost any surface.
Every time you touch a surface, the sweat from your fingertips leaves behind an invisible impression—your fingerprint. The sweat is colorless and doesn’t reveal the print, but chemicals can react with the compounds in sweat, primarily amino acids, to reveal these hidden prints. The Phase Transfer Method is unique because it uses a combination of a phase transfer catalyst and a xanthen dye to develop fingerprints.
The catalyst commonly used is tetrabutylammonium iodide, and the dye could be something like eosin Y or rose bengal.
The beauty of the Phase Transfer Method lies in its versatility. It works on both absorbent surfaces (like paper and wood) and non-absorbent surfaces (like metals, plastic, or even adhesive tape). Whether the surface is dry or wet, this method can reveal fingerprints in just a couple of minutes. For example, you can develop fingerprints on items such as paper, aluminum foil, plastic, and even wet surfaces, all in less than 2 minutes!
Step-by-step process to apply the Phase Transfer Method:
Advantages of the Phase Transfer Method
In the world of crime investigation, every little detail counts. Sometimes, the smallest clues—like a hidden fingerprint—can make all the difference. But what happens when these fingerprints are blood-stained and invisible? This is where forensic scientists use advanced techniques to reveal those critical prints, even when they seem to disappear.
When a person with blood on their fingers touches a surface, they leave behind a bloody fingerprint. While some of these prints are visible right away, repeated touches or time can make them faint or almost invisible. To solve this, forensic scientists use specialized reagents that react with the components of blood—particularly heme and proteins—to reveal those hidden prints.
Heme-Reactive Reagents
Blood is made up of two main parts: red blood cells (which contain hemoglobin) and serum. The heme in hemoglobin can react with certain chemicals to make the fingerprint visible. Let’s take a look at the reagents used in this process.
Protein-Reactive Reagents
If the fingerprint is made from the serum part of the blood (which contains proteins), then protein-reacting reagents are used. These reagents react with proteins in the blood to develop the fingerprint.
Collection and Serological Analysis
Before using these chemical reagents, it’s important to carefully collect the blood samples for serological analysis. This ensures that DNA or other vital forensic evidence can be extracted for further investigation.
Advanced Techniques
For advanced cases, forensic experts may also use the Ninhydrin-Zinc Chloride Method. This technique not only develops fingerprints but can also make them glow under laser light, allowing investigators to analyze old prints that would otherwise be hard to see.
The Cyanoacrylate Fuming Method, also known as the Superglue Technique. Cyanoacrylate fuming is a non-invasive process used to develop latent fingerprints on various surfaces. Here’s how it works: when cyanoacrylate (the main ingredient in Superglue) is heated, it releases vapors that react with the residues left behind by a fingerprint. These vapors attach to the fingerprint’s oil and sweat residues, forming a white print on the surface. The beauty of this method? It doesn’t require direct contact with the evidence, meaning there’s no risk of smudging or damaging the print!
Enhancing the Cyanoacrylate Fuming Process
While the basic process works well, there are ways to improve the efficiency and speed of this technique:
Dealing with White Prints
One challenge with the cyanoacrylate method is that the developed prints are often white, which can be hard to see on light-colored surfaces. How do we solve this? Simple – we use post-treatment techniques! For example, we can apply a Basic Yellow 40 dye, which turns the white prints into a yellow color, making them more visible. And as an added bonus, Basic Yellow 40 also glows under UV light, which is great for weak or faint prints!
Cyanoacrylate fuming isn’t just for a specific type of surface – it works on almost anything! Whether it’s absorbent or non-absorbent, light or dark, or even sticky or moist surfaces – the Superglue technique has got you covered. This includes surfaces like plastic bags, tape, wood, metals, and more!
How to Carry Out the Cyanoacrylate Fuming Process?
Here’s a step-by-step guide to using this method:
On a chemical level, the process is quite fascinating! When cyanoacrylate vaporizes, it undergoes polymerization—a process where it turns from a vapor to a solid layer. This solid layer attaches to the sweat residues left by the fingerprint, forming the visible print. The polymerization process happens much faster if there’s a base, like water or ammonium hydroxide, in the chamber, which speeds up the reaction.
Enhancing the Visibility of Prints
Although the prints are usually white, forensic experts often use fluorescent dyes or stains to make them stand out. Rhodamine 6G, for example, is a fluorescent dye that makes the prints glow under UV or laser light, making it easier to spot even the faintest fingerprints.
Vacuum Metal Deposition is a highly sensitive technique that helps reveal latent fingerprints on surfaces where other methods might struggle. It’s similar to the Cyanoacrylate Fuming Method, but instead of using superglue vapors, metal vapors are used—specifically gold and zinc.
How Does VMD Work?
The process begins by placing the object with potential fingerprints into a vacuum chamber. This chamber is emptied of air to create a controlled environment. Inside the chamber, small pieces of gold and zinc are heated until they vaporize. The procedure goes as follows:
The metals selectively bond to the fingerprint residues, and because of the difference in how the metals behave, shiny metal traces form along the ridges of the fingerprint. These metal vapors highlight the latent prints without altering the evidence. VMD is non-destructive, meaning it doesn’t interfere with DNA collection from the fingerprints, which is crucial for forensic investigations. This makes it an excellent method when other traditional techniques may compromise the evidence.
Where Can VMD Be Used?
This method can be used on a wide range of surfaces, including:
However, because it requires specialized and expensive equipment, it’s usually found in well-funded forensic laboratories.
The History of VMD
Interestingly, VMD was originally developed for industrial purposes, specifically for metal coating applications like glass mirrors. However, in 1964, Professor S. Tolansky at the University of London made an accidental discovery. While experimenting with the method, he found that the metal vapors could reveal latent fingerprints on glass surfaces. This discovery sparked further research in the UK, leading to the identification of gold and zinc as the most effective metals for fingerprint development.
Why Gold and Zinc?
The key to VMD’s success lies in the selective deposition of metals on the fingerprint residues. Here’s how it works:
This difference in how the metals behave during the deposition process is what makes VMD so powerful in revealing latent prints.
The VMD System
The VMD system consists of:
Inside the chamber, the vacuum is created, and only 2-3 milligrams of gold are used in the process. After the gold is applied, zinc is vaporized and deposited over the gold, with slight adjustments to the chamber’s pressure to ensure an even coating of zinc.
VMD’s Precision
VMD is not only precise, but it’s also effective in situations where other methods fail. For instance, it can successfully reveal fingerprints on items submerged in water for long periods, which many traditional techniques would struggle with. This makes it an indispensable tool in forensic investigations where other methods might not be applicable.
AFIS (Automated Fingerprint Identification System) is a global technology used for matching fingerprints in law enforcement and civilian applications. Its applications range from solving crimes to unlocking smartphones.
Evolution of Fingerprint Collection
AFIS Technology
Fingerprint Processing Steps
Challenges in AFIS
Matching Process
Advanced Solutions
Applications in Large-Scale Systems
Footprints are impressions left by bare feet or feet wearing footwear on surfaces such as soil, sand, snow, or dust. These impressions may be three-dimensional (indented into a substrate) or two-dimensional (surface marks).
Static Footprints: Made when a person is stationary.
Dynamic Footprints: Created during movement, providing additional information about gait and biomechanics.
Latent Footprints: Invisible to the naked eye, often requiring specialized techniques to visualize.
Photographic Documentation:
Use a high-resolution camera to photograph prints before any other action is taken.
Place a scale (e.g., ruler) adjacent to the impression for accurate measurement.
Use oblique lighting to highlight depth and texture.
Preservation of 3D Footprints:
Fixative Sprays: Use sprays to stabilize delicate prints in soft or unstable surfaces such as sand or snow.
Casting Materials:
Dental stone or plaster of Paris is used to create durable casts.
Pour the material gently to avoid disturbing the impression. Allow sufficient drying time before removal.
Preservation of 2D Footprints:
Electrostatic Lifting: A specialized device is used to lift dust-based prints onto a film.
Gel Lifters: Suitable for lifting prints from flat surfaces like tiles or glass.
Measure the dimensions of the footprints, including length, width, and arch characteristics.
Look for individualizing features such as scars, deformities, or unusual ridge patterns.
Compare with suspect footprints using overlays or software tools.
Shoe prints are impressions left by footwear, typically on surfaces such as mud, sand, carpet, or concrete. These impressions can reveal tread patterns, wear marks, and unique defects.
Visible Prints: Easily observed, such as muddy or bloody prints on a floor.
Latent Prints: Hidden and requiring chemical or physical methods for visualization.
Plastic Prints: 3D impressions in soft surfaces like mud or snow.
Photographic Documentation:
Capture high-quality images from multiple angles.
Use a tripod and oblique lighting for detailed documentation.
Lifting Techniques:
Dust Prints: Electrostatic dust print lifters.
Wet Prints: Absorb excess moisture, then lift using adhesive tapes or gel lifters.
Casting:
Use dental stone for 3D impressions. Add a layer of fixing spray to prevent collapse.
Mix casting material in a proper ratio to avoid air bubbles.
Examine the sole pattern, including size, shape, and tread designs.
Compare unique wear marks, cuts, or embedded debris.
Identify the manufacturer and model of the footwear.
Tire marks are impressions left by vehicle tires on surfaces such as roads, grass, or mud. These marks are crucial in vehicular accident investigations and crime scene reconstructions.
Yaw Marks: Curved marks made when a vehicle slides sideways while still moving forward.
Acceleration Scuffs: Caused by rapid acceleration, leaving irregular patterns.
Skid Marks: Formed when tires lose traction, often during hard braking.
Tire Impressions: 3D patterns created when tires move over soft surfaces like mud or snow.
Photography:
Take overhead images with proper scales and lighting.
Capture the entire length of the mark.
Casting:
Use sulfur or other durable materials to cast 3D impressions in soft substrates.
Ensure no air bubbles form during casting.
Documentation:
Record measurements of tread width, depth, and unique features like wear patterns or embedded debris.
Compare tread designs with tire databases to identify the make and model.
Analyze unique wear characteristics or damages.
Match impressions to specific vehicles.
Skid marks are linear or curved marks left by tires sliding across a surface during braking or turning. These marks help determine vehicle speed, direction, and braking point.
Straight Skid Marks: Indicate sudden braking in a straight line.
Curved Skid Marks: Often found during braking on a turn.
Gap Skids: Show intermittent braking, suggesting an anti-lock braking system.
Measure the length and width of skid marks.
Use formulas (e.g., speed = √(30 x distance x drag factor)) to estimate the speed of the vehicle.
Combine skid mark data with other physical evidence to reconstruct the sequence of events.
A gait pattern refers to the distinctive manner in which an individual walks. It involves analyzing footprints or shoe prints left during movement.
Stride Length: The distance between successive steps of the same foot.
Step Width: The lateral distance between left and right footprints.
Step Angle: The angle at which the foot deviates from the walking line.
Pressure Points: Areas where maximum pressure is exerted during walking.
Match gait patterns from crime scenes with CCTV footage of suspects.
Use software for biomechanical analysis to identify unique gait characteristics.
Correlate gait patterns with physical conditions (e.g., limp, injury).
Biometric identification refers to the use of physiological or behavioral characteristics of an individual to confirm their identity. Unlike traditional methods such as passwords or PINs, biometrics rely on unique and intrinsic human features, making them highly secure and nearly impossible to forge. This technology plays a crucial role in ensuring security, efficiency, and convenience in a digitally interconnected world.
These involve physical characteristics that are unique to an individual:
Fingerprint Recognition
Analyzes ridge patterns and minutiae points.
Widely used in forensic investigations, smartphones, and access control systems.
Examples: Aadhaar authentication in India, mobile phone unlocking.
Iris Recognition
Examines the unique patterns in the colored ring around the pupil.
Highly accurate with minimal error rates.
Applications: Border security, financial transactions.
Facial Recognition
Analyzes facial features like the distance between eyes, nose shape, and jawline.
Common in surveillance systems, airports, and smartphone unlocking.
Challenges: Variability due to lighting, aging, or facial expressions.
Retinal Scanning
Maps the unique blood vessel patterns in the retina.
Extremely secure but requires user cooperation.
Used in military and government applications.
Palm Print Recognition
Analyzes the ridges, creases, and lines on the palm.
Applied in law enforcement, attendance systems, and payment gateways.
DNA Analysis
Uses genetic material to identify individuals with near-perfect accuracy.
Essential in forensic investigations, ancestry tracking, and paternity tests.
These depend on patterns in human behavior:
Voice Recognition
Analyzes vocal characteristics like pitch, tone, and accent.
Used in call centers, personal assistants (e.g., Alexa, Siri), and secure banking.
Keystroke Dynamics
Studies typing patterns, speed, and rhythm.
Useful in cybersecurity for user authentication and fraud prevention.
Gait Analysis
Examines the way a person walks, considering speed, step length, and posture.
Applied in surveillance, rehabilitation, and health monitoring.
Enrollment Phase:
Biometric data is captured and stored securely in a database after preprocessing.
Example: Scanning fingerprints during Aadhaar registration.
Verification Phase:
The system matches the input biometric data with stored records to verify identity.
Example: Unlocking a smartphone with facial recognition.
Identification Phase:
The system identifies an individual by comparing the input data against multiple records.
Example: Matching crime scene fingerprints with a database.
Used in secure facilities, airports, workplaces, and data centers to control entry and restrict unauthorized access.
Example: Biometric gates at international airports.
Identification of suspects through fingerprints, DNA, and facial recognition.
Matching crime scene evidence with stored databases to solve cases.
Example: Automated Fingerprint Identification Systems (AFIS).
Patient identification to prevent fraud and ensure proper care.
Monitoring physical activity using wearable devices integrated with biometrics.
Example: Use of palm vein scanning in hospitals.
Enhances security in online transactions using fingerprints or facial recognition.
Biometric ATMs for secure and user-friendly cash withdrawals.
Example: Voice recognition for banking in call centers.
Used in national ID programs like Aadhaar in India for identification and authentication.
Facilitates secure voting systems and social welfare distribution.
Example: Biometric-enabled Public Distribution System (PDS).
Enhanced Security: Unique traits reduce the risk of impersonation, hacking, and fraud.
Convenience: Eliminates the need to remember passwords or carry physical ID cards.
Efficiency: Speeds up identity verification processes in various domains.
Non-Repudiation: Provides indisputable evidence of identity.
Scalability: Can be implemented in systems handling millions of users, such as Aadhaar.
Privacy Concerns:
Unauthorized access to biometric databases can lead to identity theft.
Ensuring data protection and compliance with privacy laws is essential.
Accuracy Issues:
Environmental factors (e.g., lighting for facial recognition), aging, or injuries may affect biometric readings.
High Costs:
Installation and maintenance of biometric systems can be expensive, especially in developing regions.
Ethical Concerns:
Use of facial recognition in surveillance raises questions about misuse and potential discrimination.
Spoofing Threats:
Despite advancements, certain biometrics like fingerprints can be spoofed using artificial materials.
Counter-Terrorism:
Helps in tracking and identifying terrorists and securing borders.
Example: Biometric screening of international travelers.
Smart Cities:
Biometric systems integrated into smart technologies for seamless identity verification in public spaces.
Example: Biometric payment systems in metro stations.
Digital Transformation:
Facilitates secure access to online services, making them indispensable in the digital age.
Example: Multi-factor authentication using biometrics for financial transactions.
Pandemic Response:
Contactless biometric systems ensure hygiene and safety during identification.
Example: Touchless facial recognition systems in workplaces.
Multimodal Systems:
Combining multiple biometric traits like fingerprints and facial recognition for increased accuracy and security.
Example: Iris and fingerprint combination for secure bank vault access.
AI and Machine Learning:
Enhancing the speed and accuracy of biometric matching and adapting to dynamic changes in traits.
Example: AI-driven facial recognition that adapts to aging effects.
Wearable Biometric Devices:
Devices like smartwatches and fitness bands for continuous monitoring and secure authentication.
Example: Smartwatches with ECG and heart rate-based authentication.
Blockchain Integration:
Securing biometric data storage and access through decentralized blockchain technology.
Example: Blockchain-based biometric voter registration.
Voice analysis involves the scientific study of human voice patterns to identify or authenticate individuals and to analyze the characteristics of speech. It is widely employed in forensic investigations, security applications, and medical diagnostics.
Voice is unique to each individual due to physiological differences in the vocal apparatus and learned speech patterns, making it a viable tool for biometric and forensic applications.
Forensic voice analysis extends beyond basic identification. It employs advanced techniques such as spectrographic analysis and signal processing to enhance recordings and provide clarity for investigative purposes.
Forensic Applications:
Identification of speakers in criminal cases (e.g., ransom calls, threat messages).
Verification of recorded conversations as evidence.
Enhancement of audio recordings to clarify faint speech or background noise.
Security and Surveillance:
Voice recognition in access control systems.
Monitoring suspect communications.
Healthcare:
Early detection of speech and voice disorders.
Diagnosis of neurological conditions affecting speech.
Linguistics and Speech Therapy:
Research on phonetics and speech patterns.
Development of personalized therapy for speech impairments.
The human voice production system comprises three primary components:
Lungs: Generate airflow required for sound production.
Trachea: Directs air from the lungs to the larynx.
Larynx: Houses the vocal cords (folds) that vibrate to produce sound.
Glottis: The space between the vocal cords; controls pitch and volume.
Oral Cavity: Includes the tongue, teeth, and lips for shaping sounds.
Nasal Cavity: Adds resonance to the voice.
Pharynx: Acts as a resonating chamber.
The combination of these systems, along with the individual’s unique anatomy, results in distinct voice characteristics.
Voice spectrography is the process of analyzing the frequency, intensity, and duration of sound waves in a visual format using a spectrogram.
A voice recording is analyzed to produce a spectrogram, which represents:
Frequency (Pitch): Measured in Hertz (Hz).
Intensity (Loudness): Represented by the darkness or color intensity in the spectrogram.
Time: Displayed along the x-axis.
Comparison of suspect voice samples with recorded evidence.
Identification of unique voice patterns such as accent, intonation, and speech rate.
Enhancement techniques to clarify audio evidence, such as reducing background noise or amplifying faint sounds, are often applied to improve the usability of recordings.
Tools like Praat, MATLAB, and iZotope RX are commonly used for voice spectrography.
Advanced forensic methods include frequency equalization, noise reduction, and waveform analysis to isolate and enhance critical parts of the recording.
Voice samples are considered biometric evidence and may be admissible in courts if collected and analyzed according to legal protocols.
Chain of custody and authentication of recordings are critical.
State v. Williams (1979): Set precedence for the admissibility of spectrographic analysis in the U.S.
Indian Evidence Act, Section 45A: Recognizes expert opinion on voice analysis.
Consent of individuals before recording their voice.
Avoidance of bias during analysis.
Ensuring no manipulation or alteration of the original evidence during enhancement processes.
Voice can be influenced by factors such as health, emotions, and environment.
Aging and vocal fatigue may alter voice characteristics over time.
Poor-quality recordings with noise or distortion may affect analysis accuracy.
Compression artifacts in digital recordings can obscure critical voice details.
Skilled mimics can imitate voices to deceive the system.
Use of voice modulation devices poses challenges.
Different forensic laboratories may use varied methodologies, leading to inconsistent results.
Training and expertise of analysts significantly affect outcomes.
Voice analysis is closely linked to broader forensic audio and video techniques. Key methods include:
Repair and Recovery:
Damaged audio recordings can often be restored using advanced tools, ensuring evidence integrity.
Evidence Enhancement:
Noise reduction, frequency filtering, and dynamic range compression are employed to make faint voices or sounds more intelligible.
Authentication:
Examiners verify the authenticity of recordings to ensure they have not been tampered with or altered.
Interpretation and Identification:
Contextual clues, such as background sounds, help investigators deduce the scene’s environment or location, aiding in case resolution.
Anything which can record or capture any event or thing so that the information is not lost. It can be written or can also be in the form of pictures and sounds.
Albert Osborne is regarded as the “Father of Document Examination.” He significantly contributed to the field by formalizing methodologies and publishing the foundational book “Questioned Documents.”
Francis Danelle: Published the first systematic treaty on document examination.
Albert Osborne: Authored “Questioned Documents,” a cornerstone text in the field.
Sulphocyanate Acid Technique: Developed by M.E. O’Neill for document analysis.
ESDA (Electrostatic Detection Apparatus): Invented by Foster, used to identify indentations in documents.
Under the Bharatiya Sakshya Adhiniyam, Section 2(1)(d), a document is defined as any matter expressed or described upon any substance by means of letters, figures, or marks intended to be used as evidence.
Stereo Microscope: Widely used for detailed document analysis, including ink interactions and impressions.
Impressions on paper result from low tensile strength and incomplete recovery.
Tools like ESDA detect indentations on documents.
Ink Properties: Ink can be liquid or semi-liquid. Indian ink is made acidic by adding ammonia (NH3).
Chromatographic Analysis: Suggested by Mitchell and Hepworth.
Common Testing Techniques: Thin Layer Chromatography (TLC) is the most frequently used.
Types of Erasers:
Chemical: Sodium hypochlorite, potassium permanganate, oxalic acid.
Physical: Standard rubbing erasers.
Restoration of Erased Areas: Achieved through fuming with ammonium sulfide or iodine.
UV Radiation for Erased Writing: Developed by Elbridge W. Stein.
Identified using ESDA for indentation marks left on paper.
Defined as a “demonstrative science.”
Writing actions are reflex actions, while shading habits are involuntary.
Guided Handwriting: Also known as assisted handwriting.
Interlineation: Writing inserted between two existing lines.
Freehand (Simulated) Forgery: Also called copy or imitation forgery.
Traced Forgery: Created by tracing over an authentic signature.
Parkinsonism: Alters natural handwriting patterns.
Governed under Section 333 BNS in Indian law.
Coin Forgery: Includes slug, cliché, and fouree techniques.
Philatelic Forgery: Involves fake stamps.
Use of holograms, microprinting, and watermarks.
Methods include actinic light and transmitted light techniques for document verification.
Reed Pen: First used in Ancient Egypt.
Quills Pen: Associated with St. Isidore of Seville.
Modern Pens:
Gel Pen: Water-based ink with a ball as the writing tip.
Ballpoint Pen: Oil-based ink with a ball tip.
Blobbing: Found in writing created with ballpoint pens.
Iron Gallotinate Ink: Also known as blue-black ink.
Sympathetic Ink: Invisible ink used for secret writing.
China Ink: The oldest liquid ink.
Earliest Ink: Made from vegetable varnish.
Fountain Pen Ink: Non-staining, air-resistant, and insoluble variants.
Washable Ink: Easily removable from fabrics.
Bleeding: Seepage of color from ink lines.
Invisible or Secret Ink: Also known as sympathetic ink.
Watermarks: Created using dandy rolls.
Washi Paper: Invented in Japan, known for durability.
Translucent Vellum: Made from the skin of newborn calves.
UV and Actinic Light: Used to identify erasures and analyze ink properties.
Grazing Angle Examination: Utilizes oblique light at 10-15° for document analysis.
Performed using transmitted light and UV radiation.
Types:
Requested standard.
Non-requested standard.
Minimum Samples: At least five sheets are required for reliable analysis.
Achieved using ammonium sulfide fuming, iodine fumes, or alcohol extraction.
Original formulations used toluene, later replaced by bromopropane due to environmental concerns.
Refers to spacing mechanisms in typewriters, often examined for authenticity.
Typescripts refer to any text produced using typewriting devices such as typewriters, modern printers, or other mechanical means. The examination of typescripts plays a crucial role in forensic investigations, primarily focusing on identifying the source device, analyzing the content for authenticity, detecting alterations, and tracing the history of document creation.
Key Examination Methods:
Identification of Typewriting Machines:
Typewriter Identification:
Evaluate typeface design, spacing, alignment, and specific defects in characters caused by wear and tear.
Analyze the pitch of typewriting (characters per inch) and ribbon types used (fabric or carbon ribbons).
Investigate line spacing irregularities and any evidence of manual adjustments.
Printer Identification:
Examine dot matrix patterns, toner distribution, and unique mechanical or software-induced artifacts.
Identify printer-specific traits such as roller marks, misalignment, or toner inconsistencies.
Analysis of Defects and Variations:
Wear and tear on type elements leading to identifiable defects such as broken or deformed letters.
Smudging or irregular ink distribution, which can be indicative of a malfunctioning or older device.
Specific patterns of damage or misalignment in repeated characters, enabling unique device identification.
Dating of Documents:
Cross-referencing typewriter or printer models with their manufacturing and usage periods.
Using chemical analysis of inks to determine the age and composition, providing insights into document chronology.
Assessing paper properties such as watermarks, fiber content, and aging effects.
Detection of Alterations:
Identifying physical alterations such as erasures, overwriting, or pasted elements.
Evaluating inconsistencies in ink color, density, or flow to detect added or modified text.
Determining the sequence of typed inputs through overlapping impressions or ink diffusion analysis.
Definition and Scope: Printed matter refers to materials produced using various printing processes, including offset printing, digital printing, intaglio, and others. Items such as currency notes, lottery tickets, and official printed documents are often scrutinized in forensic examinations for authenticity, counterfeiting, and tampering.
Key Areas of Examination:
Currency Notes:
Paper Analysis:
Study the texture, weight, and composition of paper, including embedded security features like watermarks, security threads, and fibers visible under UV light.
Detect the presence of polymer substrates in modern banknotes.
Ink Examination:
Identify specialized inks, such as magnetic inks used for machine-readable text, fluorescent inks visible under UV light, and thermochromic inks that change color with temperature.
Examine color accuracy, sharpness, and any smudging or ink bleeding.
Security Features:
Analyze microprinting, holographic patches, optically variable ink, and anti-scan patterns.
Examine advanced elements like 3D ribbons, motion effects, and color-shifting images.
Serial Number Analysis:
Verify alignment, font consistency, and ensure no duplication across genuine notes.
Lottery Tickets:
Printing Techniques:
Study unique patterns, color combinations, and fine details used by authorized printers.
Examine the printing method (e.g., flexographic or offset) for consistency with official standards.
Security Features:
Analyze tamper-proof seals, holographic overlays, and perforations designed to prevent counterfeiting.
Inspect barcodes and QR codes for accurate encoding and readability.
Validation Codes:
Confirm the integrity of printed or embedded validation codes and their compliance with official databases.
General Printed Materials:
Registration Marks:
Assess alignment and accuracy in multi-color prints to detect any discrepancies.
Paper and Ink Properties:
Evaluate the weight, texture, and composition of paper, alongside the chemical properties of inks.
Authenticity Checks:
Compare the suspect document with verified exemplars for anomalies in design, layout, and content.
Definition and Scope: Mechanical impressions are marks or prints created by devices such as seals, stamps, embossing machines, and perforating tools. These impressions serve as evidence in validating documents, detecting forgeries, and linking items to specific tools or machines.
Key Examination Methods:
Analysis of Stamps and Seals:
Design Features:
Analyze intricate patterns, textures, and inscriptions for consistency with known specimens.
Verify edge details and symmetry of the stamp impression.
Impression Depth and Consistency:
Measure the uniformity of pressure application to identify irregularities caused by tampering or misuse.
Embossed Documents:
Depth Analysis:
Use precision measuring tools to assess the depth and sharpness of embossed characters or patterns.
Alignment Checks:
Examine the spatial relationship between embossed elements and surrounding printed text to ensure proper alignment.
Wear Analysis:
Detect wear or defects in embossing dies that leave identifiable traces.
Perforations and Punched Marks:
Tool Marks:
Study the edges of perforations and punched holes for unique tool-induced characteristics.
Consistency of Patterns:
Analyze the spacing, shape, and regularity of perforations to confirm authenticity.
Dating and Source Identification:
Correlate tools or devices with their manufacturing periods to approximate the time frame of document creation.
Assess wear and damage patterns on tools to narrow down their specific usage history.
Microscopic Analysis:
High-powered microscopes reveal fine details such as fiber structure in paper, ink spread, and surface texture of mechanical impressions.
Identify micro-defects or wear in type elements and printing components.
Spectroscopic Analysis:
Techniques like UV, IR, or Raman spectroscopy allow for detailed chemical analysis of inks, pigments, and paper composition.
Detect and differentiate inks of varying ages or compositions.
Comparison with Exemplars:
Side-by-side comparison of suspect documents with genuine samples helps in identifying design anomalies, font variations, or differences in alignment.
Digital Forensics:
For digitally produced materials, analyze metadata, print logs, and embedded watermarks to trace the source and detect alterations.
Use software tools to reconstruct erased or overwritten content.
Handwriting principle:
Principles of identification of handwriting:
Influencing factors for handwriting:
Movement in writing
Pen Pressure
Shading
Standard writing/signatures are the basic requirement for ascertaining the genuineness.
Adequate writing standards:
Class of standard writing :
Collected standard:
Source of standards
Requested Standards
Forgery refers to the act of creating, altering, or imitating a document, signature, or other objects with the intent to deceive or commit fraud. The types of forgery can be categorized based on the method used. Here are the detailed explanations of the key types of forgery:
Simulated forgery, also known as freehand forgery, involves the forger attempting to replicate the genuine signature or handwriting by visually studying it. This type of forgery requires practice and skill to mimic the original style and characteristics.
Key Characteristics:
Attempts to Replicate Original:
The forger often practices extensively before executing the forgery to achieve close similarity to the original signature or text.
Indicators of Forgery:
Variations in letter formations and spacing compared to the original.
Uneven pressure or pen lifts within strokes.
Differences in natural flow or rhythm due to hesitations.
Tools for Detection:
Microscopic examination to detect tremors or unnatural pen movements.
Comparison with genuine samples to identify discrepancies in slant, speed, or consistency.
Traced forgery involves reproducing a genuine signature or handwriting by tracing over it. This can be done using methods such as light tracing, carbon tracing, or indented tracing.
Methods of Execution:
Light Tracing:
Placing the original signature under the target document and using light to trace it.
Carbon Tracing:
Using carbon paper to transfer the outline of the original signature onto the target document.
Indented Tracing:
Pressing hard over a genuine signature to create an impression, which is then traced over with a writing instrument.
Key Characteristics:
Exactness:
The traced signature often appears unnaturally similar to the original, sometimes replicating even imperfections.
Indicators of Forgery:
Consistent pen pressure without natural variation.
Overlapping lines or double strokes from repeated tracing.
Lack of fluidity in writing due to slow and deliberate tracing.
Tools for Detection:
Use of oblique lighting to identify indentations or impressions.
Examination under magnification to detect inconsistencies in ink flow and line quality.
Forgery by memory occurs when the forger relies on their recollection of the genuine signature or handwriting, without direct reference to the original.
Key Characteristics:
Memory-Based Approximation:
The forger may approximate the general style or features of the original signature based on memory.
Indicators of Forgery:
Significant deviations in complex letter formations or intricate details.
Simplifications or omissions of elements in the signature or text.
A lack of consistency with genuine handwriting samples.
Tools for Detection:
Comparative analysis with authentic samples to highlight differences.
Evaluation of stylistic inconsistencies, such as unusual slant, size, or pressure patterns.
This type of forgery occurs when an individual creates a forged signature or handwriting without access to a model. The forger either invents the signature or handwriting or uses their natural handwriting while pretending to be someone else.
Key Characteristics:
No Direct Reference:
The forgery lacks resemblance to the genuine signature or handwriting because the forger has no visual guide.
Indicators of Forgery:
Completely different style, letter formations, and slant compared to the genuine signature.
The forgery may appear more natural but inconsistent with the known writing of the impersonated person.
Tools for Detection:
Side-by-side comparison with authentic samples to identify dissimilarities.
Analysis of writing habits, such as stroke direction, speed, and letter connections.
Techniques: Forensic document examiners use a combination of methods to identify forged signatures and forgeries. Key techniques include:
Microscopic Examination:
Magnification reveals tremors, unnatural pen lifts, and inconsistencies in ink flow.
Identifies overlapping strokes, pen stops, and pressure variations.
Oblique Lighting and Indentation Analysis:
Oblique lighting highlights impressions or indentations left by tracing methods.
Detects underlying marks or pressure points that indicate a traced forgery.
Ink and Paper Analysis:
Chemical analysis of ink can reveal different types of ink used in alterations.
Examination of paper properties, such as fiber composition and watermarks, helps establish authenticity.
Comparison with Exemplars:
Genuine samples (exemplars) are compared to identify inconsistencies in letter formation, slant, and spacing.
Differences in natural variation versus repeated patterns can signal forgery.
Analysis of Writing Dynamics:
Evaluates speed, rhythm, and natural flow of writing.
A forged signature often shows hesitations, uneven pressure, or unnatural movement.
Use of Digital Tools:
Software tools analyze writing patterns, pressure sensitivity, and pen movements.
Digital forensics can also examine metadata for electronically produced signatures.
Spectroscopic Analysis:
Use advanced techniques to analyze ink composition and detect alterations or additions.
Specific Indicators in Signatures:
Tremors in Stroke:
Tremors indicate hesitations or lack of fluidity often seen in forged signatures.
Pen Pressure:
Authentic signatures show natural variations in pressure; forgeries often have uniform pressure.
Unnatural Line Quality:
Wavering or shaky lines suggest a lack of confidence in execution.
Proportionality Issues:
Inconsistent letter sizes or irregular spacing can indicate a forgery.
Baseline Deviations:
Forged signatures often fail to maintain alignment with the baseline, showing erratic shifts.
The forensic examination of credit cards and similar materials involves analyzing physical, chemical, and digital characteristics to verify authenticity, detect tampering, and gather evidence in cases of fraud, forgery, or theft. Below are detailed notes on the process:
Dimensions and Weight:
Measure the card’s dimensions (length, width, thickness) to ensure compliance with ISO/IEC 7810 standards.
Weigh the card to identify any deviations from the standard weight.
Material Composition:
Determine the primary material, usually polyvinyl chloride (PVC) or composite plastics.
Examine for signs of material layering, damage, or substitution.
Surface Inspection:
Use magnification tools to identify wear, scratches, or inconsistencies in the surface.
Inspect the edges for signs of tampering or re-lamination.
Holograms and Security Features:
Analyze holograms for clarity, alignment, and changes in appearance when tilted.
Check for microprinting, UV features, and color-shifting inks.
Microscopy:
Use a stereomicroscope to examine embossing, engraved numbers, and microtext.
Inspect the magnetic stripe for uniformity and any scratches.
Advanced Imaging:
Use infrared, ultraviolet, and laser imaging to uncover hidden security features and detect alterations.
Conduct a spectral analysis to assess the ink and material composition.
Magnetic Stripe:
Use a magnetic card reader to extract data from the stripe.
Compare the extracted data with what’s embossed on the card.
Look for evidence of erasure or overwriting.
Chip Examination:
Analyze embedded EMV (Europay, Mastercard, Visa) chips for functionality and programming.
Use specialized readers to retrieve and decode chip data.
Identify anomalies in cryptographic signatures or protocols.
Font Analysis:
Compare fonts, sizes, and alignment of printed text with known standards.
Serial Numbers:
Validate serial numbers, account numbers, and expiration dates for consistency with issuing banks.
Embossing Quality:
Inspect the raised characters for evenness and correct alignment.
Detect secondary impressions indicative of re-embossing.
Ink Analysis:
Use gas chromatography or spectroscopy to identify ink types.
Determine whether the ink matches known standards for authentic cards.
Plastic Analysis:
Perform chemical testing to verify the composition of the card material.
Use techniques like Fourier Transform Infrared Spectroscopy (FTIR) for detailed material analysis.
Data Extraction:
Extract data from the magnetic stripe, chip, or NFC (Near Field Communication) components.
Verify the extracted data against issuer records.
Code Analysis:
Decrypt digital signatures and authentication codes stored in the chip.
Tampering Evidence:
Look for anomalies such as unauthorized firmware updates or corrupted data.
Obtain reference cards from issuing institutions to:
Compare physical and digital characteristics.
Validate the authenticity of security features and printed elements.
Record all findings with high-resolution photographs and detailed notes.
Prepare a forensic report that:
Outlines methods and tools used.
Summarizes findings, including any evidence of tampering or fraud.
Provides an expert opinion on the card’s authenticity.
Fraud Investigation: Identifying counterfeit or cloned credit cards.
Identity Theft Cases: Tracing unauthorized use and origin.
Corporate Investigations: Ensuring compliance with security standards.
Law Enforcement: Supporting criminal cases involving credit card misuse.
Physical Tools: Calipers, microscopes, magnetic stripe readers, UV lamps.
Chemical Tools: Solvents, spectroscopy equipment.
Digital Tools: EMV readers, cryptographic software, forensic data extraction tools.
Chadwick sign
Jacquemier’s sign
Hegar’s sign
Palmer’s sign
Osiander’s sign
Piskacek’s sign
Ballottement
Stallworthy sign
Mcdonald’s rule
Braxton hick
Uterine souffle
Changes see in Women
|
Features | Virgin | Deflorated |
Breast | Hemispherical and firm, areola pinkish, nipple small | Pendulous, enlarged |
Labia majora | Firm, lie in apposition | Separated and flabby |
Labia minora | Pink, soft | Enlarged, pigmented, separated, peep out |
Fourchette | Intact | May be intact/torn or may show healed scar |
Fossa navicularis | Less conspicuous | Disappears |
Hymen | Intact, edges are distinct and regular with narrow opening | Usually torn (in false virgin, it may remain intact) |
Vagina | Narrow, apposed, mucosa rugose, sensitive | Wide, capacious, rugosity may partially lost |
Disorder | Cause | Features | Sub-Categories |
Organic mental disorder | Organic or lesion | Primary is due to: primary brain pathology. Secondary is due to: brain dysfunction by systemic disease | • Delirium • Dementia • Organic amnestic syndrome |
Mental and behavioral disorders | Psychoactive substance use | • Acute intoxication • Withdrawal state • Dependence syndrome | |
Schizophrenia, schizotypal and delusional disorders | Disturbances of thought, perception, affect and behaviour | • Schizophrenia • Schizotypal disorder • Persistent delusional disorder • Acute and transient psychotic disorder • Induced delusional disorder • Schizo-affective disorders | |
Mood (affective) disorders | • Mania (manic depression) • Bipolar mood disorder • Recurrent depressive disorder | ||
Neurotic, stress-related and somatoform disorders | Disturbances of emotion | ||
Behavioral syndromes | Physiological and physical disturbances | • Anorexia nervosa, bulimia(Eating disorders) | |
Behavioural and emotional disorders | Childhood and adolescence | • Hyperkinetic disorders • Conduct disorders • Tic disorders |
Features | Psychosis | Neurosis |
Severity Contact with reality Insight Personality | Major Loss of contact Absent Impaired | Minor Contact preserved Present Preserved |
Homicidal Injuries
Suicidal Injuries
Accidental Injuries
Microscopic Methods for Estimation:
Within 30 minutes:
4 Hours:
4–12 Hours:
12–24 Hours:
24–72 Hours:
3–6 Days:
10–15 Days:
Histochemical methods analyze enzymatic activity in tissues to estimate wound age based on time elapsed:
1 Hour Post-Injury:
2 Hours Post-Injury:
4 Hours Post-Injury:
8 Hours Post-Injury:
Immediate Causes of Death:
Delayed Causes of Death:
These injuries are caused by physical forces acting on the body and can be classified as follows:
Abrasion:
Contusion (Bruise):
Laceration:
Incised Wound:
Stab Wound:
Fractures:
Gunshot Wounds:
Burns:
Scalds:
Determination of Manner of Injury:
Age of Injury:
Weapon Identification:
Reconstruction of Events:
Time Since Death:
Linking Victims and Accused:
Insurance and Legal Claims:
Contact Wound:
Close-Range Wound:
Distant Wound:
Entry Wound:
Exit Wound:
Bullet Track:
Gunshot Residue (GSR):
Identification of Firearm:
Range and Direction of Fire:
Reconstruction of Crime Scene:
Linking Evidence:
Determining Intent:

iii. Temporal – 2
iii. Lacrimals- 2
vii. Vomer
viii. Mandible
SKELETAL REMAINS | ACCURACY |
Entire skeleton | 100 |
Skull + Pelvis | 98 |
Pelvis alone (best single bone) | 95 |
Skull alone | 92 |
Long bones | 80-85 |
Long bones + Pelvis | 98 |
Feature | Male | Female |
General size | Larger, more massive | Smaller, slender |
Long bones | Ridges, depression and process are more prominent. Bones of arms and legs are 8% longer | Less Prominent |
Shaft | Rougher | Smoother, thinner with relatively wider medullary cavity |
Articular surface | Larger | Smaller |
Metacarpal bones | Longer and broader | Shorter & Narrower |
Weight | 4.5 kg | 2.75 kg |
Maximum anteroposterior length of skull
Dolichocephalic (long skull) | below 75.0 |
Mesocephalic | 75.1 to 79.9 |
Brachycephalic (short skull) | 80.0 to 85.0 |
Hyper Brachycephalic | 85.1 and over |
Length
Chama Cephalic (flat skull) | 75.0 And under |
Ortho Cephalic | 70.1 to 75.0 |
Hypocephalic (High skull) | 75.1 And over |
Prognathous | 82º and under |
Mesognathous | 83º to 90º |
Hyperorthgnathous | 91º and over |
Frontal chord (frontal bone) | Nasion to bregma |
Parietal chord (parietal bone) | Bregma to lambda |
Occipital chord (occipital bone | Lambda to opisthion |
Feature | Male skull | Female skull |
General appearance | Larger, heavier, rugged, marked muscular ridges | Smaller, lighter, walls thinner, smoother |
Forehead | Receding, irregular, rough, less rounded | Vertical, round, full, infantile, smooth |
Cranial capacity | More capacious (1450-1550 cc) | Less capacious (1300-1350 cc) |
Glabella | Prominent | Less prominent |
Supraorbital/ superciliary ridge | Prominent | Less prominent |
Frontonasal junction | Distinct angulation | Smoothly curved |
Orbits | Square, rounded margins, small | Rounded, sharp margins, large |
Frontal and parietal eminence | Less prominent | Prominent |
Zygomatic arch | Prominent | Not prominent |
Occipital area (Muscle markings and protuberance) | Prominent | Not prominent |
Mastoid process | Large, round, blunt | Small, smooth, pointed |
Digastric groove | Deep | Shallow |
Condylar facet | Long, narrow | Short, broad |
Palate | Large, U-shaped, broad | Small, parabolic |
Foramen magnum | Relatively large, long | Small, round |
External auditory meatus | Bony ridge along upper border prominent | Often absent |
Feature | Caucasians | Mongoloids | Negros |
Orbits | Triangular | Rounded | Square |
Nasal Openings | Narrow and elongated | Rounded | Broad |
Sagittal Contour | Arched | Arched | Flat |
Palate | Triangular or V-shaped | Rounded or horseshoe shaped | Rectangular or broad U-shaped |
INDEX | FORMULA | MALE | FEMALE |
Washburn/Ischiopubic index | Length of pubis × 100 Length of ischium | 73-94 | 91-115 |
Sciatic notch index | Width of sciatic notch × 100 Depth of sciatic notch | 4-5 | 5-6 |
Sternal index | Length of manubrium × 100 Length of body | 46.2 | 54.3 |
Corporo Basal index | Breadth of body of 1st sacral vertebra × 100 Breadth of base of sacrum | > 42 | < 42 |
Sacral index | Transverse diameter of base of sacrum × 100 Anterior length of sacrum | < 114 | > 114 |
Kimura’s Base-wing index | Width of wing (ala of sacrum) × 100 Width of base transverse diameter of body of S1 | ||
Chilotic line index | Sacral part of chilotic line × 100 Pelvic part of the chilotic line
Chilotic line:
| ||
Feature | Male pelvis | Female pelvis |
General appearance | Massive, rougher, prominent muscular markings | Less massive, slender, smoother, muscular markings not prominent |
Shape | Deep funnel | Flat bowl |
Preauricular sulcus | Not frequent, narrow, shallow | More frequent, broad, deep |
Obturator foramen | Large, oval, base upwards | Small, triangular, apex forwards |
Iliac Crest | More Prominent | Less Rounded |
Acetabulum | Large, 52 mm diameter | Small, 46 mm diameter |
Sacrum | Large, Uniform Curvature, Promontory more prominent | Small, Upper half straight, Lower half curved forward, Promontory less prominent |
Sacroiliac articulation | Large, extends to 2½-3 vertebrae | Small, extends to 2-2½ vertebrae |
Greater sciatic notch | Narrow, deep, small | Broad, shallow, large |
Iliopectineal line | Well-marked, rough | Rounded, smooth |
Ischial tuberosity | Inverted | Everted |
Body of pubis | Narrow, triangular | Broad, square, pits on posterior surface, if borne children |
Subpubic angle | V-shaped, sharp angle, 70°-75° | U-shaped, rounded, broader angle, 90°-100° |
Pelvic brim or inlet | Heart-shaped | Circular or elliptical shaped |
Pelvic cavity | Conical, funnel shaped | Broad, round |
Pelvic outlet | Smaller | Larger |
Coccyx | Less movable | More movable |
Ischio-pubic index | 73 to 94 | 91 to 115 |
Sciatic notch index | 4 to 5 | 5 to 6 |
Feature | Male mandible | Female mandible |
General size | Larger and thicker | Smaller and thinner |
Chin | Square | Rounded |
Body height | At symphysis greater | At symphysis smaller |
Ascending ramus | Greater breath | Smaller breath |
Angle of body and ramus | Less obtuse (under 125º); prominent. and everted | More obtuse, and not prominent |
Condyles | Larger | Smaller |
Mental tubercle | Large and Prominent | Insignificant |
Feature | Mandible in Infancy | Mandible in Adult | Mandible in Old age |
Body | Shallow | Thick and long | Shallow |
Ramus | Short, oblique, forms obtuse angle with body | Less obtuse angle | Obtuse angle with body (about 140°) |
Mental foramen | Opens near the lower margin and directed forwards | Opens midway between upper and lower margins and directed horizontally backwards | Opens near the alveolar margin |
Condyloid process | At a lower level than coronoid process | Elongated and projects above coronoid process | Neck is bent backwards |
Feature | Male vertebral column | Female vertebral column |
Atlas, breadth | 7.4 to 9.9 cm | 6.5 to 7.6 cm |
Length of vertebral column | 73 cm | 60 cm |
Corporo-basal index of sacrum | 45 | 40.5 |
Feature | Male thorax | Female thorax |
General | Longer and narrower | Shorter and wider |
Sternum | Body longer and more than twice the length of the manubrium; upper margin is in level with lower part of the body of second thoracic vertebra; breadth more. Length more than 149mm. | Shorter and less than twice than the length of manubrium; upper margin in level with lower part of the body of third thoracic vertebra; breadth less. Length less than 149mm. |
Ribs | Thicker, lesser curvature and are less oblique | Thinner; greater curvature, and more oblique |
Clavicle | Longer, broader, heavier, less curved | Smaller, narrow, lighter, more curved |
Feature | Male femur | Female femur |
Head | Larger and forms about 2/3 of a sphere. Vertical diameter more than 47mm | Smaller and forms less than 2/3 of a sphere. Vertical diameter less than 45mm. |
Neck | Obtuse angle with the shaft about 125º | Less obtuse angle with the shaft |
Bicondylar width | 74 to 89 mm | 67 to 76 mm |
Angulation of shaft with condyles | Around 80º | Around 76º |
Indices of long bones
INDEX | FORMULA | VALUE IN RACE |
Brachial index | Length of Radius × 100 Length of Humerus | Europeans: 74.5 Negroes: 78.5 |
Crural index | Length of Tibia × 100 Length of Femur | Indians: 86.5 Negroes: 86.2 Europeans: 83.3 |
Humerofemoral index | Length of Humerus × 100 Length of Femur | Europeans: 69 Negroes: 72.4 |
Intermembral index | Length of Humerus + Radius × 100 Length of Femur + Tibia | Europeans: > 70 Negroes: < 70.5 |
Racial Differences in the Femur
Criteria & Propounder | Asian Origin | European Origin | African Origin |
Anterior Curvature (Stewart & Trudell) | straighter | more curved | straighter |
Proximal Diaphysis Shape (Gilbert & Gill) | anteroposterior flattening | rounder | rounder |
Intercondylar Notch Depth (Baker et al.) | undetermined | shallower | deeper |
Bergmann’s Rule | Body mass increases in inhabitants of colder climates | ||
Allen’s rule | Extremities increase in length in warmer climates | ||
Holliday and Falsetti’s | Discriminant Function Coefficients | ||
Duray | C3–C6 spinous processes have higher frequency of bifidity in whites than in blacks | ||
Feature | Male scapula | Female scapula |
Height | More than 157 mm | Less than 144 mm |
Glenoid cavity | Height greater | Height less |
Laws of ossification
Exception – Carpal bones ossification occur after birth. And Clavicle has 2 primary centres of ossification.
Exception – Lower end of femur. The secondary centre for the lower(distal) end appears before birth in the 9th month of fetal life. It fuses with the shaft between the 16th and 18th years.
Exception – Lower end of fibula. A secondary centre for the upper end appears in the 3rd or 4th year; and fuses with the shaft between the 17th and 19th years.A secondary centre for the lower end appears in the first year; and fuses with the shaft between the 15th and 17th years.
Exception – Upper end of femur. 3 secondary centres appear at the upper end of femur, one each for the head (1st year), the greater trochanter (4th year), and the lesser trochanter (around the 12th year). Each centre fuses independently with the shaft in the reverse order of appearance: the lesser trochanter at about 13 years, the greater trochanter at about 14 years, and the head around 16 years
Centers of bones | Appearance | Fusion |
Clavicle – Medial end Sternum Manubrium Body • Ist segment • IInd segment • IIIrd segment • IVth segment Xiphoid process | 15-19 years 5 month IUL
5 month IUL 7 month IUL 7 month IUL 10 month IUL 3 years | 20-22 years 60-70 years
14-25 years from below upwards 3rd and 4th-15 years 2nd & 3rd-20 years 1st & 2nd-25 years >40 years with the body |
Humerus (upper end) • Head • Greater tubercle • Lesser tubercle |
1 year. All three unite at 6 years 3 years 5 years |
18 years 4-5 years with head 5-7 years with greater tubercle |
Humerus (Lower end) • Medial Epicondyle • Capitulum • Trochlea • Lateral Epicondyle |
5-6 years 1 year 9-10 years 10-12 years |
16 years Capitulum,trochlea & L. epicondyle form conjoint tendon at 14 years,unites with shaft at 15 years |
Radius • Upper end • Lower end |
5-6 years 1-2 years |
15-16 years 18-19 years |
Ulna • Upper end • Lower end • Head Ist metacarpal • Head other metacarpals |
8-9 years 5-6 years 2 years 1½ to 2½ years |
16-17 years 18-19 years 15-17 years 15-19 years |
Hip bone • Triradiate cartilage • Iliac crest • Ischial tuberosity • Sacrum |
11-13 years 14-15 years 15-16 years 8 months IUL |
14-15 years 18-20 years 20-22 years 25 years |
Femur (Upper end) • Head • Greater trochanter • Lesser trochanter Femur (Lower end) |
1 year 4 years 14 years 9 month IUL |
17-18 years 17 years 15-17 years 17-18 years |
Tibia • Upper end • Lower end |
9 month IUL 1 year |
16-17 years 16 years |
Fibula • Upper end • Lower end |
4 years 1 year |
17-19 years 15-17 years |
Scapula • Coracoid base • Acromion process |
10-11 year 14-15 year |
14-15 years 17-18 years |
Hand bones | Foot bones | ||
Type of bone | Age of ossification | Type of bone | Age of ossification |
Capitate Hamate Triquetral Lunate Scaphoid Trapezium Trapezoid Pisiform | 2 months 3 months 3 year 4 year 5 year 6 year 7 year 9-12 years | Calcaneus Talus Cuboid Lateral cuneiform Medial cuneiform Intermediate cuneiform Navicular | 5 months 7 months 10 months 1 year 2 years 3 years 3 years |
SITE FOR X-RAY (REGION) | AGE (YEARS) | |
MALE | FEMALE | |
Elbow | 15-16 | 13-14 |
Wrist | 18-19 | 16-17 |
Shoulder | 18-19 | 17-18 |
Iliac crest | 19-21 | 18-19 |
Ischial tuberosity and inner end of clavicle | 21-23 | 21-22 |
Suture | Fuses after | |
Lateral and occipital fontanelle | 2 months after birth | |
Posterior fontanelle | 6-8 month of birth | |
Anterior fontanelle | 1½-2 years of birth | |
Metopic suture | 2-4 years but may extend upto six years | |
Basioccipital and basisphenoid | 18-20 years | |
Suture | Starts Closing | Closes by |
Sagittal | 25 years | 35-40 years |
Coronal | 25-30 | 40 years |
Lambdoid | 25-35 years | 45-50 years |
Pterion | 40 years | 65 years |
Mastoid-occipital | 45 years | 80 years |
Asterion | 80 years | |
Parieto-temporal | 70 years | |
Bertillon System of Identification
History
Facial reconstruction is of two types:
Steps of Facial Reconstruction
iii. Buccinator
Hyoid Bone
ossification of hyoid bone
I. Hyoid bone fuses from 6 centres.
II. Ossification begins in greater Cornu at 10 lunar months of intrauterine life.
III. Ossification in lesser cornu begins at 16 years
Cremains refers to the study of Cremated remains
Triquetral bone
iii)The triquetral bone ossifies between 9 months and 50 months
iv)formerly k/as cuneiform bone
v)It connects with the pisiform, hamate, and lunate bones
Sauvergrain & Nahum method is based on scoring : Elbow bones
Harris lines are :
(1) Lines of arrested growth in bones
Matiegka’s formula is used for :
(1) Calculating the skeletal weight from stature
Wormian bone
I.It is an extra bone piece that is present within a suture in the Cranium.
III. Also known as Inca bone
Steele and McKern’s formula : Calculation of a person’s height from fragmented bones
(1) Dupertuis and Hadden’s formula
(2) Trotter and Gleser formula
(3) Karl Pearson formula
Proportional relationship of size of various body parts with stature is known as Allometry
Identification an individualization from teeth
Forensic odontology: branch of dentistry deals with proper handling and examination of dental evidence with proper evaluation and presentation of dental findings in interest of justice
Methods applied in forensic odontology
History
In India
Famous notorious figures identified by forensic odontology
Dental identification
Factors for dental identification
Teeth formation
In utero
After birth
Temporary teeth | Eruption time in months | Complete root calcification in years |
Central incisors lower | 6-8 | 1½- 2 |
Central incisors upper | 7-9 | 1½- 2 |
Lateral incisors upper | 7-9 | 1½- 2 |
Lateral incisors lower | 10-12 | 1½- 2 |
First molar | 12-14 | 2- 2½ |
Canines | 17-18 | 2- 2½ |
Second molar | 20-30 | 3 |
Neonatal lines
Age | Number of teeth |
1 | 8 |
1½ | 16 |
2- 2½ | 20 |
Mixed dentition
In adults
Permanent teeth | Eruption time in years | Complete root calcification in years |
First molar | 6-7 | 9-10 |
Central incisors | 6-8 | 10 |
Lateral incisors | 8-9 | 11 |
First premolars | 9-11 | 12-13 |
Second premolars | 10-12 | 13-14 |
Canines | 11-12 | 13-14 |
Second molar | 12-14 | 14-16 |
Third molar | 17-21 | 18-25 |
Difference between temporary and permanent teeth
Temporary teeth | Permanent teeth |
Small narrow light and delicate | Big broad heavy and strong |
Crowns China white in colour | Crowns ivory white in colour |
Junction of crown with fang often marked by ridge | Junction of crown with fang are not so marked |
Edges serrated | Edges not serrated |
Neck more constricted | Neck less constricted |
Anterior teeth vertical | Anterior teeth usually inclined somewhat forward |
Molars are large | Premolars which replace temporary molars are usually small |
Crown are flat and their roots are smaller and more divergent | Crowns have cusp which are sharp roots are bigger and straight |
Methods of age identification
Gustafson’s method
DALIZ METHOD
LAMENDOLA’S METHOD
BOYDE’S METHOD
Special Features of Some Races
RH = root height
P = (periodontitis height ÷ RH) × 100,
T = (root dentine translucency height ÷ RH) × 100
Taxa | Flies | Beetles | ||||||||
Phylum | Arthropoda | Arthropoda | ||||||||
Class | Insecta | Insecta | ||||||||
Order | Diptera
| Coleoptera
| ||||||||
Family | • Calliphoridae • Sarcophagidae • Sepsidae • Piophilidae • Phoridae • Muscidae • Fannidae • Sphaeroceridae | • Carrion beetles (Silphidae) • Rove beetles (Staphylinidae) • Clown beetles (Histeridae) • Trogid beetles (Trogidae) • Hide and skin beetles (Dermestidae) • Checkered (or bone) beetles (Cleridae) • Sap-feeding beetles (Nitidulidae) • Ground beetles (Carabidae) | ||||||||
Genus & Species | • Lucilia sericata • Calliphora vicina • Calliphora vomitoria |
Process in Corpse Decomposition :
Rate of decomposition is temperature dependent
Time of body decomposition to a skeleton, in relation to temperature
Y = 1285 / X |
Y = number of days to mummification, or skeletonization
X= average temperature for the days before the body was found
Stages of post mortem changes : By Goff
On Land
Stage 1: Fresh stage.
Stage 2: Bloated Stage.
Stage 3: Active decay stage.
Stage 4: Post-decay stage.
Stage 5: Skeletonization.
Submerged in water
Floating decay stage.
Family | Example | Description |
Calliphoridae | Blowfly (Blue or Green Bottlefly) |
|
Sarcophagidae | Flesh Fly |
|
Muscidae | House Fly |
|
Piophilidae | Cheese skippers |
|
Family | Example | Description |
Silphidae | Carrion, Burying, Sexton beetles |
|
Staphylinidae | Rove beetles |
|
Carabidae | Ground beetles |
|
Dermestidae | Skin beetles |
|
Histeridae | Clown & Hister beetles |
|
Tenebrionidae | Darkling beetles |
|
Cleridae | Checkered & Ham beetles |
|
Scarabidae | Hide beetles |
|
Types of Feeders